摘要
目的观察脐带间充质干细胞(umbilical cord mesenchymal stem cells,UC-MSCs)治疗异丙基肾上腺素致心力衰竭(heart failure,HF)大鼠对心肌组织Toll样受体4(Toll-like receptor 4,TLR4)表达的影响,并初步探讨其可能作用机制。方法雄性SD大鼠60只,空白组16只不做处理正常饲养;剩余大鼠44只用异丙基肾上腺素建立心力衰竭大鼠模型,32只心力衰竭大鼠制模成功,随机分为对照组(n=16)和干细胞移植组(n=16)。在治疗前和治疗后1个月,应用流式细胞仪检测外周血单核细胞表面TLR4的表达,检测血清肿瘤坏死因子αlpha(tumor necrosis factor-α,TNF-α)水平和N末端脑利钠肽前体(N terminal pro-brain natriuretic peptide,NT-proBNP)。结果治疗后对照组、移植组的TLR4的表达、TNF-α和NT-proBNP水平均较本组治疗前显著降低(P<0.05),移植组的下降值显著高于对照组(P<0.05);TLR4表达与血清TNF-α水平呈正相关(P<0.05)。结论 UC-MSCs可显著降低TLR4和TNF-α的表达,改善心力衰竭症状。
Objective To explore the changes of toll-like receptor 4 （TLR4） expression in myocardium cells in the rats with heart failure after transplantation of human umbilical cord mesenchymal stem cells （UC-MSCs）.Methods Sixty 813 male rats were divided into 3 groups： the sham group （n = 16）, control group （n = 16）, and transplantation group （n = 16）. The concentration of tumor necrosis factor αlpha （TNF-α） and N terminal pro-brain natriuretic peptide （NT-proB- NP） in the serum were measured by enzyme-linked immunosorbent assay （ELISA）.The expression of TLR4 in peripheral blood mononuclear cells was detected by flow cytometry. Results The TLR4, TNF-α and NT-proBNP of control group and transplantation group after the treatment were lower than that before the treantmet （P〈0.05）,but the decline in the transplantation group was greater than that in the control group （P 〈0.05）. There was positive correlation between the level of TLR4 and TNF-α expression in myocardium （P〈0.05）.Conclusion UC-MSCs can down regulate the expression of TLR4, and improve heart failure symptoms.
出处
《华南国防医学杂志》
CAS
2015年第12期879-881,895,共4页
Military Medical Journal of South China
基金
广西壮族自治区自筹基金项目(Z2013542)
