摘要
The objective of this paper is to design a based on composite membrane with certain mechanical porous polyvinyl alcohol （PVA） strength and biocompatibilities serving as tissue regenerative scaffolds. PVA-glycosaminoglycan （GAG）-type I collagen （COL） composite membrane was fabricated by PVA with different molecular weight （Mw） and alcoholysis degree （AD） being blended with certain amounts of GAG and COL and dried at 38~C for 24 h. The water content of the composite membranes were from 61.9% to 95.1% and swelling ratio ranged from 123.6% to 621.7%. Scanning electron micro- scope （SEM） analysis proved that PVA-GAG-COL composite membrane has porous and homogenous structure. Biocompatibility test results showed that the composite membrane was nontoxic, which could promote adhesion and proliferation of fibroblasts on the com- posite membrane. In conclusion, PVA-GAG-COL composite membrane with high water content and swelling ratio, suitable mechanical strength and good biocompatibility, has potential in tissue engineering and regenerative medicine.
The objective of this paper is to design a porous polyvinyl alcohol(PVA)based on composite membrane with certain mechanical strength and biocompatibilities serving as tissue regenerative scaffolds.PVA-glycosaminoglycan(GAG)-type I collagen(COL) composite membrane was fabricated by PVA with different molecular weight(Mw)and alcoholysis degree(AD) being blended with certain amounts of GAG and COL and dried at 38℃ for 24 h.The water content of the composite membranes were from 61.9%to 95.1% and swelling ratio ranged from 123.6% to 621.7%.Scanning electron microscope(SEM) analysis proved that PVA-GAG-COL composite membrane has porous and homogenous structure.Biocompatibility test results showed that the composite membrane was nontoxic,which could promote adhesion and proliferation of fibroblasts on the composite membrane.In conclusion,PVA-GAG-COL composite membrane with high water content and swelling ratio,suitable mechanical strength and good biocompatibility,has potential in tissue engineering and regenerative medicine.
基金
National Natural Science Foundation of China,grant number:51070853
Guangzhou Technology Plans,grant number:2010B103315 and 2010J-E041
