不同类型表面材料对单增李斯特菌生物被膜形成的影响

The effect of different surface materials on the formation of Listeria monocytogenes biofilms

以常见的食源性病原菌单增李斯特菌为研究对象,研究PPR,PVC,玻璃,不锈钢4种常见食品加工设备材料对生物被膜态微生物的影响,分别采用扫描电镜观察和超声振荡分离、平板计数培养检测不同类型材料表面生物被膜的形成与生长.实验结果表明:35℃下TSB培养24 h,单增李斯特菌可在PPR,PVC,玻璃和不锈钢等材料表面形成稳定的生物被膜.其表面粘附的菌体数目由多到少依次为:不锈钢>PPR>PVC>玻璃.这说明材料粗糙度对生物被膜的形成影响显著,且粗糙度越大,越易形成生物被膜.改善材料的表面性质来减少菌体的粘附可作为一种间接控制生物被膜的新途径.

Scanning electron microscopy,ultrasonic vibration isolation,culture surface plate count were employed in the investigation of the impact of PPR,PVC,glass,stainless steel on the formation and growth of common foodborne pathogen Listeria monocytogenes bacteria biofilms. The results showed that Listeria monocytogenes could form stable biofilms on the surface of PPR,PVC,glass,and stainless steel with TSB culturing 24 h at 35 ℃. The bacteria number adhering to the surface was from more to less in the order：stainless steal PPR PVC glass. This illustrated that roughness of the material played a great role in the formation of biofilm. The rougher the surface,the easier the formation of the biofilm. Improving the surface properties of materials to reduce the adhesion of bacteria could be used as a kind of new way of indirect control skin membrane.