大麦赤霉病抗性种质的挖掘及其小孢子对DON毒素胁迫培养的响应

Evaluation of the Resistance of Barley Varieties(Lines) to Fusarium Head Blight and Responses of Isolated Microspores to Deoxynivalenol Stress

采用单花滴注法将禾谷镰刀菌分生孢子接种42份大麦品种(系),对其赤霉病菌的抗性进行评价,并利用DON毒素对抗性不同大麦的小孢子进行离体胁迫培养。结果表明,供试品种(系)对赤霉病抗性存在明显差异,可将其划分为高抗、中抗、中感和重感四种类型。在5 mg·L^(-1) DON毒素的胁迫下,抗性不同的大麦小孢子的愈伤组织相对产量为高抗>中抗>高感,表明供试品种小孢子水平的耐DON毒素能力与植株水平的抗赤霉病能力之间存在一定的联系。

The Fusarium head blight（FHB） resistance of different barley varieties（lines） were investigated by single floret injection. The results showed that there were significant differences among barley varieties（lines）for FHB resistance, which could be categorized into four groups： highly resistant, moderately resistant, moderately susceptible and highly susceptible. Responses of barley isolated microspores cultured by deoxynivalenol（DON） treatment were further investigated. The relative callus yields from isolated microspores of barley varieties（lines） with different levels of FHB resistance were obviously different. When cultured in induction medium supplemented by 5 mg·L^（-1） DON, highly resistant line had the highest yield, followed by moderately resistant line, and highly susceptible had the lowest yield. These results suggested that the response of cultured microspores to DON stress had correlation with FHB resistance.