miR-195表达调控对大肠癌细胞HT-29增殖和凋亡的影响及其机制探讨

Effect of miR-195 on Proliferation and Apoptosis of Colorectal Cancer Cell Line HT-29 and Its Mechanism

目的:探讨miR-195对大肠癌细胞HT-29增殖和凋亡的影响及可能的机制。方法:应用脂质体转染的方法,将miR-195 mimics转染入HT-29细胞,q RT-PCR检测转染后miR-195的表达,分别采用CCK-8法及流式细胞术检测细胞增殖和凋亡情况,qRT-PCR和Western blot检测转染后VEGFA mRNA和蛋白表达变化。结果:与NC组比较,miR-195 mimics转染组明显上调HT-29细胞miR-195的表达,抑制细胞增殖,促进细胞凋亡,下调VEGFA mRNA及蛋白表达,两组比较差异均有统计学意义(P<0.05)。结论:上调HT-29细胞的miR-195表达,可明显抑制细胞增殖、促进细胞凋亡,其机制可能与抑制其靶基因VEGFA表达相关。

Objective：To investigate the effect of miR-195 on proliferation and apoptosis of colorectal cancer cell line HT-29 and its mechanism.Method：MiR-195 mimics was transfected into HT-29 cells by lipofectamine 2000. The miR-195 expression was measured by real-time quantitative reverse transcriptase-polymerase chain reaction（qRT-PCR）,cell proliferation and apoptosis were respectively evaluated by CCK-8 assay and flow cytometry.VEGFA mRNA and protein were assessed by qRT-PCR and Western blot. Result：miR-195 mimics group was significantly increased miR-195 expression in HT-29 cells,inhibited cells proliferation and increased cells apoptosis and downregulation of mRNA VEGFA and protein expression,compared with the NC group,the differences were statistically significant（P〈0.05）. Conclusion：Overexpression of miR-195 in HT-29 cells may significantly suppress cells proliferation and induce cells apoptosis,probably via downregulating its target gene VEGFA expression.