大孔树脂分离纯化槟榔花多酚研究

Separation and purification of polyphenols from areca inflorescence by macroporous resin

采用大孔吸附树脂对槟榔花多酚进行分离纯化,确定其分离纯化条件。通过静态吸附试验和动态吸附解吸试验,考察AB-8、SP700、SP850、XAD-7HP、D101和HP2MG等6种型号树脂对槟榔花多酚的吸附量和解吸率,筛选出吸附效果最好的树脂,并得出最佳的吸附条件。结果表明,AB-8树脂的吸附和解吸效果最好,可以用准二级动力学方程较好地描述AB-8树脂对槟榔花多酚的吸附。最佳的分离纯化条件为:槟榔花多酚粗提液p H为4,上样初始浓度为0.4 mg/m L,洗脱剂乙醇浓度为60%,上样流速和洗脱流速均为3 BV/h。AB-8型大孔树脂在所确定的工艺条件下,树脂的吸附—解吸附性能稳定,且能较好地分离纯化槟榔花多酚,多酚纯度在纯化前为2.7%、纯化后为34.6%。

Macroporous resins were used to separate and purify polyphenols from the extract of areca inflorescence, and the conditions of separation and purification were determined. The adsorption capacity and desorption rate of six kinds of resins, SP850, XAD-7HP, SP700, D101 and HP2MG, were compared to select the most suitable one, through static adsorption experiment and dynamic adsorption and desorption experiment. The results showed that AB-8 resin was the best one and the kinetic data fitted the pseudo-second-order kinetics model better. Optimal conditions were as follows： the sample pH was 4.0, concentration of ethanol used as elution agent was 60%, initial sample concentration was 0.4 mg/mL, adsorption rate and desorption rate was 3 BV/h. AB-8 resin performed great in separating and purifying polyphenols from areea inflorescence, and the purification of polyphenols was 2.7% at first and increased to 34.6% after purification.