TRAPPC9基因对奶牛金葡菌乳房炎抗性性状的遗传效应

The Genetic Effect of TRAPPC9 on Mastitis Resistance to S.aureus in Dairy Cows

旨在分析奶牛金黄色葡萄球菌(金葡菌)抗性候选基因TRAPPC9的遗传效应。本研究利用中国北方地区6个牛场517头具有完整DHI、年龄及胎次记录的中国荷斯坦牛的生产数据,使用Multiplex SnaPshot微测序技术分析TRAPPC9基因4个SNPs的基因型,利用特异性PCR技术检测牛只感染金葡菌情况,同时测定血清中TNF-α、IFN-γ、IL-17、NF-κB等细胞因子的含量。在此基础上,利用GLM模型最小二乘法分析TRAPPC9基因4个SNPs对奶牛金葡菌乳房炎抗性的遗传效应。结果表明:IL-17、TNF-α和IFN-γ可作为有效的乳房炎参考指标。对于金葡菌阴性牛,TRAPPC9基因SNP4(C2477531T)对SCS有极显著影响(P<0.01);对于金葡菌阳性牛,该SNP对IL-17有极显著效应(P<0.01)。TRAPPC9基因可作为中国荷斯坦牛金葡菌乳房炎抗性分子标记之一。

The present study was designed to investigate the genetic effect of TRAPPC9 gene on resistance to Staphylococcus aureus（S.aureus）in Chinese Holstein cattle.A total of 517 Chinese Holstein cows with intact data of DHI,age and parity were collected from 6farms of North China.Multiplex SnaPshot was used to analyze the genotype of 4SNPs of TRAPPC9 gene,the specific PCR was used to detect S.aureus infecting cattle.Meanwhile,the contents of IFN-γ,IL-17,NF-κB and TNF-αin serum for each cattle were measured.Based on this,the genetic effects of the4 SNPs in TRAPPC9 on mastitis resistance to S.aureus were analyzed by the least squares method of GLM model.The results indicated that IL-17,TNF-αand IFN-γcould be used as effective indicators for mastitis.With regard to the S.aureus negative Chinese Holstein cows,SNP4（C2477531T）of TRAPPC9 had highly significant effect on SCS（P〈0.01）,while the SNP showed a highly significant effect on IL-17 in S.aureus positive cows（P〈0.01）.These data imply that bovine TRAPPC9 gene could be a powerful molecular marker of mastitis resistance to S.aureus in Chinese Holsteins.