大通牦牛胎盘与胎儿组织线粒体SDH基因分析

The Succinate Dehydrogenase Genetic Analysis of Placenta and Fetus Tissue Mitochondria in Datong Yak

旨在探讨高原低氧环境下大通牦牛发育早期线粒体标志酶-琥珀酸脱氢酶(SDH)在不同组织中的表达模式。本研究以大通牦牛妊娠4月龄牦牛及出生1日龄犊牦牛为研究对象,采用实时荧光定量PCR技术对牦牛胎盘组织,4月龄胎儿和出生1日龄犊牦牛的心肌、骨骼肌、肝和大脑组织中SDHA、SDHB、SDHC和SDHD 4个亚基mRNA定量表达进行测定,研究不同组织线粒体中SDH mRNA表达量与其组织器官能量代谢的相关性。结果显示:出生1日龄犊牦牛心肌组织线粒体SDHA、SDHB、SDHC和SDHD 4个亚基mRNA表达量均显著高于4月龄胎儿;4月龄胎儿大脑组织线粒体SDHA、SDHB、SDHC和SDHD 4个亚基mRNA表达量均显著高于出生1日龄犊牦牛。综上表明:SDH基因表达模式表现为4月龄胎儿大脑组织和出生1日龄犊牦牛心肌组织中SDHA、SDHB、SDHC和SDHD 4个亚基转录水平均表现为上调表达,表明4月龄胎儿大脑组织和出生1日龄犊牦牛心肌组织能量代谢调控活动旺盛。

The expression patterns of mitochondrial marker-succinate dehydrogenase（SDH）in different tissues of Datong yak at early development stage were investigated under the plateau hypoxia environment.Pregnant yaks at 4-month-old and yaks at 1-day-old were sampled,genetic mRNA expression quantities（SDHA,SDHB,SDHCand SDHD）of placental tissues,cardiac muscle,skeletal muscle,liver and brain tissues of fetuses at 4-month-old and yaks at 1-day-old were determined using qRT-PCR,the correlation between mitochondrial SDH mRNA expression qualities and energy metabolism in different organs and tissues were researched.The results showed that cardiac muscle mitochondrial genetic mRNA expression quantities（SDHA,SDHB,SDHCand SDHD）of yaks at 1-day-old were significantly higher than those of fetuses at 4-month-old,cerebral mitochondrial genetic mRNA expression quantities（SDHA,SDHB,SDHC and SDHD）of fetuses at 4-month-old were significantly higher than those of yaks at 1-day-old.SDH gene expression patterns suggested that gene（SDHA,SDHB,SDHCand SDHD）transcription levels were up-regulated expression between the brain of fetus at 4-month-old and cardiac muscle of yak at 1-day-old,which was reflected on exuberant energy metabolism regulation activities between the brain of fetus at 4-month-old and cardiac muscle of yak at 1-day-old.