黄鳝性腺发育过程中Amh和SOX9基因的表达分析

Expression analysis of Amh and SOX9 in gonads development during sex reversal in rice field eel

文章研究了黄鳝性腺发育过程中抗苗勒氏管激素(anti-Mullerian hormone,Amh)基因和Sry相关高泳动类非组蛋白基因9(Sry-related high mobility group-box gene 9,SOX9)2个基因的表达量变化及关系,进而推断其基因表达通路,为研究黄鳝性逆转过程中基因的调控理论奠定基础。文章提取黄鳝Ⅰ龄卵巢(Ⅱ期卵巢)、Ⅲ龄卵巢、Ⅱ龄间期性腺早期、Ⅱ龄间期性腺后期、Ⅲ龄精巢5个时期的性腺组织RNA并反转录成cDNA,通过半定量和荧光定量聚合酶链式反应(polymerase chain reaction,PCR)对其表达量进行定量分析。结果表明,Amh和SOX9基因在黄鳝性腺发育过程中的表达没有特异性,随着卵巢的败育和精巢的发育,表达量明显升高。Amh基因在Ⅲ龄精巢表达有显著增高,SOX9基因的表达量高于Amh(Ⅲ龄精巢例外)。Amh和SOX9基因的表达呈现正相关,后期Amh基因的表达量显著升高可能是由于存在一个诱导SOX9基因表达的信号,两者在黄鳝性逆转过程及精巢的发育过程中存在一定的协同关系,可能存在Amh—SOX9基因信号通路。

The expression level and relationship between two genes i. e. anti-Mullerian hormone（Amh） and Sty-related high mobility group-box gene 9 （SOXg） in gonads development during sex reversal in the rice field eel were investigated, and t;e gene expression pafhway was ：concluded, The study laid foundation for the genes regulation theory during the sex reversal of the rice field eel. The cDNA of five gonadal samples including one-year-old ovary（ovary of stage II ）, three-year-old ovary, two-year- old early ovotestis, two-year-old later ovotestis, and three-year-old testis ＂were reversely transcribed ;rom the extracted RNA. The expression of the two genes in each stage of the gonads was analyzed by using semi-;tuantitative polymerase chair/reaction（PCR） and real-time fluorescence quantitative PCR. It was showed.that the expression of Amh and SOX9 had no specificity during gonad development, with the ovary abortiveness and testisdevelopment,the transcription level significantly increased. The expression of Amh had a marked increase in three-year-old testis; the expression of SOX9 was higher than that of Amh except in three-year-old testis. It came to the conclusion that the expression of Amh and SOX9 may have positive correlation, the markedly increased expression of Amh in three-year-old testis may be a signaling pathway to induce the later expression of soxg, which had a certain synergy relationship during the process of sex reversal and development of the testis in the rice field eel, which may be the gene chain like Amh-SOX9 in Oryzias latipes.