急性髓细胞白血病p73基因启动子区域异常甲基化的研究

Study of aberrant p73 promoter methylation in patients with acute myeloid leukemia

1目的探讨急性髓细胞白血病(Acute Myeloid Leukemia,AML)患者p73启动子CpG岛的甲基化状况及其临床意义。2方法采用甲基化特异性聚合酶链反应(MS-PCR)检测56例AML患者骨髓单个核细胞p73启动子区域甲基化率,同时以30例缺铁性贫血患者作为对照组。χ2检验分析其甲基化程度与临床参数之间的关系。3结果AML组p73甲基化率26%,正常对照组3%,两组比较差异有统计学意义(P<0.05)。异常核型急性髓细胞白血病(karyotypically abnormal AML,KA-AML)组p73甲基化率43%,显著高于正常核型急性髓细胞白血病(cytogenetic normal AML,CN-AML)组(14%),两组比较差异有统计学意义(P<0.05)。AML低危组p73甲基化率为60%、AML中危组为21%、AML高危组为33%,3组间比较差异无统计学意义(P>0.05)。4结论 AML患者p73甲基化显著高于对照组,提示p73可能参与AML的发生发展;p73在KA-AML中更易发生启动子区域的甲基化;p73甲基化可能不足以成为AML独立的预后指标。

Objective To study the methylation status of p73 promoter in patients with acute myeloid Leukemia and explore its significance with clinical data.Methods Methylation of p73 promoter in bone marrow cells from 56 AML patients and 30 iron deficiency anemia controls were detected by methylation spfecific PCR（MSP）.The changes of p73 methylation status were measured and analyzed with correlated clinical data by chi-square test.Results The prevalence of DNA methylation of p73 gene in AML patients was significantly higher than that in control group （26%vs3%, P〈0.05）,The prevalence of DNA methylation of p73 in karyotypically abnormal AML （43 %） was significantly higher than that in cytogenetic normal AML（14%）（ P 〈0.05） while there was no statistical difference of p73 methylation frequency was seen among the three groups of the low risk group （60G）, mediumrisk group （21%） and high--risk groups of AML （33%） （ P 〉0.05）.Conclusion p73 gene metbylation was significantly higher than the control group,suggesting that p73 gene may be involved in the development of AML. p73 promoter region was more susceptible to methylate in the KA--AML than CN--AML.p73 methylation may not be AML independent prognostic indicator.