酵母提取物对成纤维细胞的作用及抑制黑素生成的研究

Effect of yeast extract on fibroblasts proliferation and melanin inhibition

通过探索米酒来源酵母提取物(WAE)对成纤维HS68细胞和黑素瘤B16细胞存活率的影响以及对黑素瘤B16细胞黑素生成的作用,研究WAE的安全性及功效性。结果表明,WAE对成纤维HS68细胞和黑素瘤B16细胞的安全质量分数为不高于0.10%;与空白对照组(不含WAE)相比,WAE的质量分数为0.10%和0.01%时,成纤维HS68细胞的存活率分别为106.36%和109.33%,说明WAE在安全质量分数范围内具有促进成纤维HS68细胞增殖的功效;与空白对照组相比,WAE的质量分数为0.10%,0.05%和0.01%时,黑素瘤B16细胞的黑素合成抑制率分别为68.32%,51.68%和19.77%,说明WAE在安全质量分数范围内有抑制黑素瘤B16细胞黑素生成的作用。与相同质量分数的啤酒来源日本酵母提取物(A)和国产酵母提取物(B)相比,WAE的细胞安全性与黑素抑制作用效果更优。

Via the exploration of effect of the yeast extract that originated from rice wine production（ WAE） on viability of fibroblast HS68 cells as well as its action for formation of melanin by melanoma B16 cells,the safety and efficacy of the WAE were examined. The results showed that,the safety WAE mass fraction for HS68 and B16 is ≤0. 10%. As comparing with the control group,when mass fraction of WAE is 0. 10% and 0. 01%,it promotes HS68 cells proliferation to 106. 36% and 109. 33% respectively. The experiments demonstrated that under the safe concentration,WAE displays the effect of promoting proliferation of HS68 cells. WAE can inhibit68. 32%,51. 68% and 19. 77% of melanin formation when the WAE mass fraction is 0. 10%,0. 05% and0. 01% respectively. The experiments demonstrated that under the safety mass fraction,WAE displays the effect of inhibiting melanin formation of B16 cells. As comparing with Japanese beer yeast extract（ A） and domestic beer yeast extract（ B） with the same mass fraction,the safety and efficacy of WAE are superior to that of A and B.