HLA-G慢病毒表达载体的构建及病毒颗粒的包装

Construction of lentiviral vector carrying HLA-G gene and packing of the lentiviruses

设计合成全长人类白细胞抗原G（HLA-G）基因系列,在克隆质粒pLV-EF1a-EGFP-N的基础上构建HLA-G基因慢病毒表达载体,测序鉴定pLV-EF1a-EGFP-N-HLA-G构建成功。与包装质粒（pGag/Pol、pRev、pVSV-G）转染293T包装细胞,包装出高效感染率的慢病毒颗粒,测定病毒滴度为1×109TU/ml。HLA-G慢病毒表达载体的构建及病毒颗粒的包装为转染HLA-G诱导移植肾免疫耐受的研究奠定了基础。

The human leucocyte antigen-G （HLA-G）gene was synthesized. According to gene sequencing, the lentiviral vector carrying HLA-G was constructed successfully on the basis of cloning plasmid pLV-EF1 a-EGFP-N. The constructed lentiviral vector was transferred into 293T package cell with pGag/Pol, prey and pVSV-G and the lentiviruses with high infection efficiency were produced. The virus titer was 1 x 109 TU/ml. Construction of lentiviral vector carrying HLA-G gene and packing of the lentiviruses laid the material foundations for the study of HLA-G gene in inducing immune tolerance of transplanted kidney.