干扰素对尖锐湿疣患者免疫应激及HMGB1、MIF、GM-CSF的影响

Research on influence of interferon on immune stress and HMGB1,MIF,GM-CSF in patients with condyloma acuminatum

目的:观察干扰素治疗尖锐湿疣的疗效,及其对患者免疫应激、HMGB1、MIF、GM-CSF表达的影响。方法:将240例尖锐湿疣患者随机分为两组,对照组和观察组各120例,所有患者均采用CO2激光法进行疣体清除,观察组患者在此基础上应用干扰素治疗。比较两组间疗效、复发情况差异,比较治疗前后两组患者间T细胞亚群变化及血清炎症细胞因子、HMGB1、MIF、GM-CSF变化。结果:观察组治愈率高于对照组,观察组复发率低于对照组,差异均具有统计学意义(P<0.05);治疗后3、6个月观察组CD4^+、CD4^+/CD8^+大于对照组,CD8^+低于对照组;IL-2及IFN-γ水平明显高于对照组,而IL-4、IL-10水平则低于对照组;血清HMGB1、MIF、GM-CSF水平低于对照组,各项指标组间差异有统计学意义(P<0.05)。结论:干扰素能调节T淋巴细胞亚群,抑制炎症反应,改变机体的免疫应激状态,从而有利于提高CA患者的治愈率、降低其复发率。

Objective： To observe the therapeutic effect of interferon in condylorna acuminatum and its influence on im mune stress and high mobility group protein-1 （HMGB1）, maerophage migration inhibition factor （MIF）, Granuloeyte-macro phage clone stimulating factor （GM-CSF） in patients with condyloma acuminatum. Methods：A total of 240 patients with con- dyloma acuminatum were randomly divided into control group and observation group, with 120 cases in each group. CO2 laser was used for the removal of warts in all patients, while patients in observation group were treated with interferon after that. Then the differences in curative effect and the recurrence between the two groups were compared, and the ehanges of T eell subsets and serum inflammatory cytokines, HMGB1, MIF, GM-CSF in two groups were compared before and after treatment. Results：The cure rate of observation group was significantly higher than that of control group and the recurrence rate of the observation group was significantly lower than that of the control group （P 〈0.05）. About 3, 6 months after treatment, the CD4＋ and CD4＋ / CD8＋ in observation group were significantly higher than those in control group, and CD8＋ was significantly lower than that in control group; the serum levels of IL-2 and IFN-Twere higher than control group, while the IL-4, IL-10 were lower than control group; the serum levels of H MGB1, MIF,GM-CSF were lower than control group as well, and the difference between groups was statistically significant（P〈0.05）. Conclusions：Interferon can regulate T lymphocyte subsets, inhibit inflammatory reaction and change the immune stress state, so as to improve the cure rate and reduce the recurrence rate of condyloma aeuminatum patients.