原头蚴对体外培养小鼠脾细胞中Th细胞亚群的影响

Effect of protoscolex on T subsets in mice spleen cells in vitro

目的:观察原头蚴对体外培养小鼠脾细胞中Th细胞亚群及相关细胞因子表达的影响。方法:BALB/c小鼠脾细胞与原头蚴共培养,用ELISA检测上清液IL-4、IFN-γ、TGF-β含量;同时收集细胞用FCM检测Th细胞亚群。结果:ELISA检测不同感染时间原头蚴组IL-4、TGF-β分泌均增加,与对照组相比差异有统计学意义(P<0.05);FCM检测原头蚴组不同时间点Th2、Treg细胞的比例,差异均有统计学意义(P<0.05),但Th1细胞不同时间点组间差异无统计学意义(P>0.05)。结论:原头蚴能刺激体外培养的小鼠脾细胞向Th2、Treg细胞分化且上调相应细胞因子的分泌,提示这些亚群细胞可能在包虫病感染的免疫逃逸中发挥一定作用。

Objective：To observe the effect of protoscolex on Th subsets and correlative cytokine in mice spleen cells in vitro. Methods： Co-culture spleen cells from BALB/c mice with protoseolices,then IL-4, IFN-y and TGF- β production in cell culture supernatants were analyzed by ELISA. The percentage of Tb subsets were detected by Flow Cytometry analysis. Results： Secretion levels of IL-4 and TGF-13 were significantly increased in spleen cells at different time point in co-culture system with protoscolices. Ratios of Th2 and Treg cells were also significantly increased in co-culture system at differeht time points than the control groups. However, there was no statistical significance for ratio of Tbl cells at different time points. Conclusion ： The protoscolex can increase the ratios of Tb2 cells and Treg cells from spleen cells. Secretion levels of IL-4 and TGF-β were also increased in spleen cells co-cultured with protosco- lices. The resuhs suggest that these Th cell subsets play a role in the immune escape of the hydatid disease.