葡萄球菌肠毒素超抗原广谱抑制性多肽的设计及空间结构研究

Study on design and three-dimension structure of a broad-spectrum inhibitory peptide against staphylococcal enterotoxins superantigen

目的:以SEs氨基酸高度保守序列作靶序列设计抑制性多肽,研究筛选出的多肽P72的空间结构。方法:用生物信息学软件"Vector NTI 10.3,Insight II 2000,Discovery Studio 1.7"等分析及预测多肽P72的空间结构。结果:P72在SEA、SEB和SEC的同源序列在空间结构具有高度的相似性,P72远离SEB的TCR和MHCⅡ结合位。结论:P72可能不是与MHCⅡ类分子及TCR结合而产生的抑制作用,其具体的抑制机制有待深入研究。

Objective ：Peptides were designed on the basis of high conservative regions of amino acid sequences and structures of the SEs, three-dimension structure of P72 was constructed. Methods： Bioinformatics analysis softwares such as Vector NTI 10. 3, InsightII 2000, Discovery Studio 1.7 were used to analyse and predict the space structure of P72. Results ： three-dimensional domains of the peptide P72 from SEA, SEB and SEC were quite similar, Peptide P72 was far away from TCRVβ chain and MHC class II molecule. Conclusion： The inhibitory activity of peptide P72 may not due to binding to MHC Ⅱ and TCRVβ chain. The exact mechanism of inhibitory activity of P72 should be explored.