攀枝花市致倦库蚊幼虫对常用杀虫剂的抗性研究

Resistance of Culex pipiens quinquefasciatus larvae to commonly used insecticides in Panzhihua city,Sichuan

目的了解四川省攀枝花市致倦库蚊幼虫对6种常用杀虫剂的抗性状况,为科学使用杀虫剂提供依据。方法2014年从攀枝花市5个县(区)采集幼虫,用WHO推荐的幼虫浸渍法,测定4龄期幼虫的半数致死浓度(LC50)。结果攀枝花市致倦库蚊幼虫除对仲丁威较敏感(R/S<1)外,对高效氯氰菊酯、氯菊酯、溴氰菊酯均产生不同程度的抗性,抗性倍数依次分别为75.00、12.40和2.50倍;对双硫磷、敌敌畏的抗性倍数分别为1.67和1.65倍;对仲丁威的抗性倍数<1。结论在灭蚊工作中应采取综合性防制措施,科学合理使用杀虫剂。

Objective To investigate the resistance of Culex pipiens quinquefasciatus larvae to six insecticides and direct future application of insecticides. Methods Mosquito larvae were collected in 2014 from 5 counties （districts） of Panzhihua city, and tested by WHO recommended larval dipping method for medium lethal concentration. Results Culex pipiens quinquefasciatus larvae collected from Panzhihua city were susceptible to fenobucarb （R/S〈 1）, but showed various degrees of resistance to cypermethrin, permethrin and dehamethrin. The resistance ratio of Cx. pipiens quinquefasciatus larvae was 75.00 fold to beta cypermethrin, 12.40 fold to permethrin 2.50 fold to deltamethrin. Slight tolerance was indicated to temephos and DDVP where resistance ratios were 1.67 and 1.65 respectively. Conclusion It was necessary to apply integrated measures and use insecticides strategically in controlling mosquitoes.