自体乳酸杆菌体外增殖后再回植在恢复阴道微生态中的应用研究

Study on the application of replantation of autologous lactobacillus after in vitro proliferation in restoration of vaginal microecology

目的探讨自体残留产过氧化氢乳酸杆菌在体外增值后再回植技术在恢复阴道微生态中的效果。方法对26例存在阴道微生态失调的妇女进行自体乳酸杆菌体外增殖后再回植,移植后1个月、3个月、6个月、12个月分别取阴道分泌物对阴道微生态进行评估。结果移植后12个月观察对象阴道内白细胞数量明显下降(Hc=18.17,P=0.000<0.05),作为优势菌的革兰氏阳性大肠杆菌菌群密集度(Hc=24.22,P=0.000<0.05)明显增加,在观察期共有9例阴道炎复发,其中真菌性阴道炎复发7例。结论自体残留产过氧化氢乳酸杆菌体外增值后再回植技术在恢复阴道微生态失调方面疗效明显,长期定植和增殖效果好,但对真菌无明显抑制作用。

Objective To explore the effect of replantation technique of autologous residual hydrogen peroxide laetobacillus after in vitro proliferation on restoration of vaginal microecology. Methods Replantation of autologous residual hydrogen peroxide lactobacillus after in vitro proliferation was conducted among 26 women with vaginal microecological imbalance, vaginal secretions were obtained after 1,3, 6, and 12 months of replantation to evaluate vaginal micro-balance. Results Vaginal WBC count after 12 months of replantation decreased sig- nificantly （Hc= 18. 17, P=0. 000〈0. 05）, the concentration of Gram-positive bacilli bacteria increased as the dominant bacteria （Hc= 24. 22, P = 0. 000〈0. 05 ） ; vaginitis recurred in 9 cases, including recurrent fungal vaginitis in 7 cases. Conclusion The curative effect of replantation technique of autologous residual hydrogen peroxide lactobacillus after in vitro proliferation on restoration of vaginal microecology is significant, the effects of long-term plantation and proliferation are good, but it has no obvious inhibiting effect on fungi.