高压氧联合rh-EPO对窒息新生大鼠心肌细胞caspase-3表达的影响

Effect of hyperbaric oxygen combined with rh-EPO on Caspase-3 expression in myocardial cells of neonatal rats with asphyxia

目的 探讨高压氧联合rh-EPO对窒息新生大鼠心肌细胞caspase-3表达及细胞凋亡的影响,为临床治疗新生儿窒息后心肌损害提供分子理论依据。方法 将新生3~7 d的健康SPF级Wistar大鼠随机分为对照组、窒息组、rh-EPO组、高压氧组、高压氧联合rh-EPO组,每组8只,分别给予相应的干预措施后,采用免疫组织化学染色法检测Caspase-3蛋白表达水平,采用TUNEL染色检测心肌细胞的凋亡率。结果 与rh-EPO组和高压氧组比较,高压氧联合rh-EPO组可明显降低窒息新生大鼠心肌细胞caspase-3的表达和细胞凋亡,差异有统计学意义（P〈0.05）。结论 新生大鼠窒息后采用高压氧及rh EPO联合治疗可明显减轻窒息新生大鼠心肌细胞的损害。

Objective To explore the effect of hyperbaric oxygen combined with recombinant human erythropoietin （rh-EPO ） on Caspase-3 expression and apoptosis of myocardial cells in neonatal rats with asphyxia, provide a molecular basis for clinical treatment of my- ocardial damage after neonatal asphyxia. Methods Healthy specific-pathogen free Wistar rats aged 3-7 days were randomly divided into control group, asphyxia group, rh-EPO group, hyperbaric oxygen group, hyperbaric oxygen combined with rh-EPO group, 8 rats in each group. The rats in different groups were treated by corresponding measures. Immunohistochemical staining was used to detect the expression levels of Caspase-3 protein. TUNEL staining was used to detect apoptotic rate of myocardial cells. Results Compared with rh-EPO group and hyperbaric oxygen group, the expression level of Caspase-3 protein and apoptotic rate of myocardial ceils in hyperbaric oxygen combined with rh-EPO group decreased, thcre were statistically significant differences （ P〈0. 05 ） . Conclusion Hyperbaric oxygen combined with rh-EPO can significantly relieve damage of myocardial ceils in neonatal rats after asphyxia.