携氧抗休克液对清醒大鼠重度失血性休克中血乳酸和碱剩余变化的影响

Effect of oxygen- carrying shock resuscitation fluid on blood lactate and base excess changes in severe hemorrhagic shock of unrestrained conscious rats

目的观察携氧抗休克液对清醒大鼠重度失血性休克复苏过程中血乳酸和剩余碱变化的影响,以评估其对失血性休克复苏的作用。方法 24只SD大鼠在清醒状态下经股动脉释放其全身总血量的65%,制作重度失血性休克模型,随机分为3组,每组8只,即生理盐水(NS)组、万汶组(羟乙基淀粉130/0.4)和携氧抗休克液组(万汶+HBOCs)组。分别在休克前,休克末、复苏后及复苏后2 h,抽取血液进行血乳酸(LD)、剩余碱(BE)的测定。结果休克后,LD浓度明显高于休克前(P<0.01)。复苏后,三组大鼠LD均有所下降,携氧抗休克液组下降最明显,万汶组次之,NS组最差,差异有统计学意义(P<0.01);复苏2 h后,三组大鼠LD均有所下降,携氧抗休克液体组下降最明显,基本恢复到休克前水平(P>0.05);万汶组次之,NS组最差,差异有统计学意义(P<0.05)。结论携氧抗休克液体能明显的降低重度失血性休克大鼠动脉血乳酸含量,改善碱剩余,减轻代谢性酸中毒,保护组织细胞,减少无氧代谢所造成的损伤。

Objective To investigate the effect of oxygen -carrying shock resuscitation fluid on blood lactate and base excess changes in unrestrained conscious rats with severe hemorrhagic shock（SHS）,in order to assess its role in hemorrhagic shock resuscitation. Methods Twenty -four SD rats awake were released 65%of its total blood volume through the femoral artery for building SHS model in unrestrained conscious rats,which were randomized into 3 groups：NaCl （NS）group,6%HES group and oxygen -carrying shock resuscitation fluid group.Lactic acid and base excess were detected before shock,at end stage of shock,at end stage of recovery and 2 hours after recovery.Results After shock,LD was significantly higher than before shock（P 〈0.01）.After recovery,LD of the three groups of rats had declined,which declined most significantly in oxygen -carrying shock resuscitation fluid group,followed by HES group and NS group.The difference was statistically significant （P 〈0.01）.Two hours after recovery,LD levels of oxygen -car-rying shock resuscitation fluid group decreased most obviously,and recovered to the level before shock （P 〉0.05）,and there was sig-nificant difference between the other two groups （P 〈0.05）.Conclusion Oxygen -carrying shock resuscitation fluid can significant-ly reduce arterial blood LD in the SHS of rats,improve BE,reduce metabolic acidosis and protect tissue cells.