ova和HPV E_6及其蛋白滴鼻免疫增强小鼠抗体水平

ova,HPV E_6 and its protein can enhance antibody levels by intranasal co-immunization in mice

[目的]探讨ova和HPV E_6及其蛋白通过滴鼻共免疫的方式提高抗体水平的有效性。[方法]选择ova和OVA蛋白,HPV E_6和HPV E_6蛋白滴鼻共免疫ICR小鼠,检测免疫后小鼠血清中的Ig G、Ig G1、Ig G2a和s Ig A,生殖道黏液中s Ig A的表达水平。[结果]ova及其蛋白共免疫组血清中的抗体Ig G及其亚类、血清和生殖道洗液中的s Ig A与对照组相比差异极显著(P<0.01),初免后第28 d,血清中的Ig G抗体滴度达到28万。HPV E_6与其蛋白共免疫组血清中的抗体Ig G及其亚类、生殖道洗液中的s Ig A与对照组相比差异极显著(P<0.01),初免后第35 d,血清中的Ig G抗体滴度达到25万。[结论]用ova和HPV E_6及其蛋白通过滴鼻共免疫,产生高水平抗体,共免疫组的抗体水平比基因疫苗组增强了近百倍,比蛋白免疫组增强了2.5倍,表明这种免疫方式能够有效激发黏膜免疫反应和系统免疫反应。

[ Objective ] The aim of study is to explore if it would induce high antibody responses by intranasal co -immuniza- tion with ova ,HPV E6 and their proteins. [ Methods] ova and OVA protein ,HPV E6 and HPV E6 protein were chosen as target antigen, chitosan as a carder, and the ICR mice were immunized by intranasal route. IgG, IgG1 , IgG2＋, slgA antibody levels in serum andslgA in vaginal washing were detected through indirect ELISA. [ Results ] The significant improvement of IgG, IgG subclasses, slgA can be detected in ova and OVA protein group （ P 〈 0.01 ）, the titer of 28 days after 1 st immunization is 280, 000. The significant improvement of IgG, IgG subclasses, slgA can be also observed in HPV E6 and HPV E6 protein group （ P 〈 0.01 ） ,the titer of 35 days after 1st immunization is 250,000. [ Conclusion] The antibody level was significantly enhanced with ova,HPV E6 and their proteins by co - immunization through intranasal route, The antibody level of co - immunization in- creaseed in hundred times and 2.5 times than gene group and protein group, The results show that this approach can effectively stimulate the mucosal and system immune response.