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CXCR4在乳腺癌组织芯片中的表达及其临床意义

Expression of CXCR4 in Breast Cancer Tissue Microarray and Its Clinical Significance
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摘要 目的检测趋化因子受体4(CXC chemokinereceptortype4,CXCR4)在各种乳腺癌组织及相应正常乳腺组织中的表达并分析其临床病理意义。方法组织芯片中标本收集于2010年5月—2014年9月,应用免疫组化法检测组织芯片中204例乳腺癌组织和36例正常乳腺组织CXCR4蛋白的表达,以棕色颗粒为阳性标准计算染色强度和阳性细胞占总细胞数的百分比进行半定量处理。结果 CXCR4蛋白主要表达于细胞膜和细胞浆,在相应正常乳腺组织、小叶原位癌、导管内原位癌和浸润性导管癌中的阳性表达率依次升高,分别为8.3%(3/36)、25.0%(1/4)、10.0%(5/50)、72.0%(108/150),4组间差异有统计学意义(P<0.001);在I、II和III级以上分级的乳腺癌组织中,CXCR4蛋白的阳性表达率分别为18.2%(2/11)、80.4%(78/97)、69.8%(30/43),3组间差异有统计学意义(P<0.001);CXCR4蛋白在有转移的肿瘤组织和无转移的乳腺癌组织中的阳性率分别为88.4%(61/69)和11.1%(15/135)。结论 CXCR4在部分乳腺癌组织中高表达,且与乳腺癌组织类型及乳腺癌组织侵袭转移能力密切相关。 Objective To detect the expression of CXC chemokine receptor 4 （CXCR4） in various breast cancer tissues and normal breast tissues and analyze its clinical pathological significance. Methods Immunohistochemistry was used to detect the expression of CXCR4 protein in 204 cases of breast cancer tissues and 36 cases of normal breast tissues in the tissue microarray samples collected from May 2010 to September 2014. Using brown granules as the positive standard, staining in-tensity and percentage of positive cells in the total cell number were calculated for semi-quantitative treatment. Results CXCR4 protein was mainly expressed in cell membrane and cytoplasm. The positive expression rate of CXCR4 in normal breast tissues, lobular carcinoma in situ, intraductal carcinoma in situ, infiltrating ductal carcinoma was gradually increased, which was respectively 8.3%（3/36）, 25.0%（1/4）, 10.0%（5/50） and 72.0%（108/150）, and there was a significant difference be-tween these 4 groups（P〈0.001）. The positive expression rate of CXCR4 protein in the breast cancer tissues of stage I, II, III and over III was 18.2%（2/11）, 80.4%（78/97） and 69.8%（30/43）, respectively, there was significant difference between the 3 groups （P〈0.001）. The positive rate of CXCR4 protein in metastatic tumor tissues and non-metastatic breast cancer tissues was 88.4%（61/69） and 11.1%（15/135）, respectively. Conclusion CXCR4 is highly expressed in some breast cancer tissues, and is closely related to the type of breast cancer and the invasion and metastasis of breast cancer.
作者 张杨 王成昆 彭波 朱欢 张志杰
机构地区 南华大学医学院肿瘤研究所 广州医科大学附属肿瘤医院肿瘤研究所
出处 《中外医疗》 2015年第33期14-16,共3页 China & Foreign Medical Treatment
关键词 CXCR4 乳腺癌 组织芯片 免疫组织化学法 CXCR4 Breast cancer Tissue microarray Immunohistochemistry
分类号 R5 [医药卫生—内科学]
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参考文献13

  • 1Zeelenberg IS,Ruuls-Van SL,Roos E.The chemokine receptor CXCR4 is required for outgrowth of colon carcinoma micrometastases[J].Cancer Res,2003,63(13):3833-3839.
  • 2Platt D,Amara S,Mehta T,et al.Violacein inhibits matrix metalloproteinase mediated CXCR4 expression:potential anti-tumor effect in cancer invasion and metastasis[J].Biochem Biophys Res Commun,2014,455(1-2):107-112.
  • 3Douglass S,Meeson AP,Overbeck-Zubrzycka D,et al.Breast cancer metastasis:demonstration that FOXP3 regulates CXCR4 expression and the response to CXCL12[J].J Pathol,2014,234(1):74-85.
  • 4hang Z,Ni C,Chen W,et al.Expression of CXCR4 and breast cancer prognosis:a systematic review and meta-analysis[J].BMC Cancer,2014(14):49.
  • 5Oliveira FV,Rubie C,Ghadjar P,et al.Changes in CXCL12/CXCR4-chemokine expression during onset of colorectal malignancies[J].Tumour Biol,2011,32(1):189-196.
  • 6Hinton CV,Avraham S,Avraham HK.Role of the CXCR4/CXCL12 signaling axis in breast cancer metastasis to the brain[J].Clin Exp Metastasis,2010,27(2):97-105.
  • 7Akashi T,Koizumi K,Tsuneyama K,et al.Chemokine receptor CXCR4 expression and prognosis in patients with metastatic prostate cancer[J].Cancer Sci,2008,99(3):539-542.
  • 8Ehtesham M,Stevenson CB,Thompson RC.Preferential expression of chemokine receptor CXCR4 by highly malignant human gliomas and its association with poor patient survival[J].Neurosurgery,2008,63(4):820.
  • 9Wang J,Wang J,Sun Y,et al.Diverse signaling pathways through the SDF-1/CXCR4 chemokine axis in prostate cancer cell lines leads to altered patterns of cytokine secretion and angiogenesis[J].Cell Signal,2005,17(12):1578-1592.
  • 10Tamamura H,Hori A,Kanzaki N,et al.T140 analogs as CXCR4 antagonists identified as anti-metastatic agents in the treatment of breast cancer[J].FEBS Lett,2003,550(1-3):79-83.

二级参考文献35

  • 1Muller A. Homey B. Sara H. et al. Involvement of chemokine re-ceptors in breast cancer metastasis[J]. Nature. 2001. 410( 6824) : 50-56.
  • 2Chu QD. Panu L. Holm NT. et al. High chemokine receptor CXCR4 level in triple negative breast cancer specimens predicts poor clinical outcome[J]. Surg Res. 2010.159(2) :689-695.
  • 3Parker CC. Kim RH. Li BD. et al , The chemokine receptor CX-CR4 as a novel independent prognostic marker for node-positive breast cancer patients[J]. J Surg Oncol.2012 .106(4) :393-398.
  • 4Hiller DJ. Meschonat C. Kim Rv et al v Chemokine receptor CX-CR4 level in primary tumors independently predicts outcome for patients with locally advanced breast cancer[J]. Surgery. 2011. 150(3) :459-465.
  • 5Sacanna E. Ibrahim T. Gaudio M. et al. The role of CXCR4 in the prediction of bone metastases from breast cancer: a pilot study[J]. Oncology.2011.80(3-4) :225-23l.
  • 6Jiang J. Thyagarajan-Sahu A. Loganathan J. et al. Breast De-fend? prevents breast-to-lung cancer metastases in an orthotopic animal model of triple-negative human breast cancer[J]. Oncol Rep.2012.28(4):1139-1145.
  • 7Kobayashi T. Tsuda H. Moriya T. et al, Expression pattern of stromal cell-derived factor-I chemokine in invasive breast cancer is correlated with estrogen receptor status and patient prognosis [J]. Breast Cancer Res Treat. 2010.123(3) :733-745.
  • 8Salvucci O. Bouchard A. Baccarelli A. et al. The role of CXCR4 receptor expression in breast cancer: a large tissue microarray study[J]. Breast Cancer Res Treat. 2006.97(3) : 275-283.
  • 9Guerreiro R. Santos-Costa Q. Azevedo-Pereira JM. The chemo-kines and their receptors: characteristics and physiological Iunc-tions[J]. Acta Med Port.2011.24(4) :967-976.
  • 10Sierro Fv Biben Cv Martinez-Munoz Lv et al. Disrupted cardiac de-velopment but normal bcrnatopoiesis in mice dificient in the sec-ond CXCLl2/SDF-l receptor. CXCR7 [J]. Pore Natl Acad Sci U S A.2007 .104(37): 14759-14764.

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中外医疗
2015年 第33期

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