摘要
以玉米螟(Pyrausta nubilalis)为对象筛选出一株高毒力生防菌株苏云金芽孢杆菌(Bacillus thuringiensis)NBIC380。通过培养基优化,获得适宜的培养基配方D。补料发酵研究的结果表明,固定氮源浓度为80 g/L,发酵前期补料效果明显,补料速率为根据发酵不同阶段进行调整,可以取得更好的发酵结果,有利于提高产量,降低生产成本。
A biocontrol strain Bacillus thuringiensis NBIC380 with high virulence to Pyrausta nubilalis was obtained. Accord-ing to this strain, appropriate media formulations D was obtained by medium optimization. Fed-batch fermentation experiments showed fermentation pre-feeding effect was obvious by using 80 g/L nitrogen concentration. Feeding rate was adjusted accord-ing to the different stages of fermentation. This strategy could improve production and reduce production costs.
出处
《湖北农业科学》
2015年第24期6248-6251,共4页
Hubei Agricultural Sciences
基金
湖北省农业科技创新中心项目(2013NKYJJ21)
国家科技支撑计划项目(2011BAD40B01)
