猪TCAP基因转录因子的筛选与鉴定

Screening and Identification of Transcription Factor of Pig TCAP Gene

克隆了猪TCAP基因1 662 bp的启动子序列,序列分析发现其启动子区域存在Myo D、Myo G、MEF2转录因子结合位点,并构建了3个转录因子超表达载体,分别与TCAP基因启动子载体共转染PK细胞。结果表明,3个转录因子使TCAP基因启动子活性升高,均正调控TCAP基因的表达。

In this study,1 662 bp sequence of pig TCA P gene promoter was cloned. Some specific transcription factor （MyoD, MyoG and MEF2） binding sites were found in TCA P gene promoter by bioinformatics analysis. Over-expression vectors of MyoD,MyoG and MEF2 were successfully constructed and transfected with promoter sequences, respectively. Detection analysis showed that TCA P promoter activity was significantly higher. The results indicated that transcription factors MyoD,MyoG and MEF2 could bind to the TCA P promoter sequences, and enhance its activity.