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猪链球菌2型1910RR蛋白多克隆抗体的制备 被引量：1

Preparation of Polyclonal Antibody of Streptococcus suis Type 2 1910 Response Regulator Protein
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摘要 通过原核表达系统表达了猪链球菌2型(Streptococcus suis type2,SS2)1910RR蛋白,并制备了多克隆抗体。结果表明,猪链球菌2型1910RR重组蛋白以上清形式表达,Western-blot检测证实了1910RR蛋白具有良好的免疫学活性,1910RR多克隆抗体效价为1∶4,为进一步研究1910RR蛋白功能奠定了良好基础。 The polyclonal antibody of 1910RR protein of Streptococcus suis type 2 was expressed and prepared. The results showed that 1910RR protein was expressed in the supernatant and has good immunological activity. The polyclonal antibodies of rabbit anti 1910RR protein was 1：4. The preparation of polyclonal antibody was successful and can be used for function research of 1910RR protein.
作者 程尧 田永祥 刘泽文 周丹娜 段正赢 杨克礼 郭锐 刘威 袁芳艳
机构地区 湖北省农业科学院畜牧兽医所 长江大学动物科学学院
出处 《湖北农业科学》 2015年第24期6320-6323,共4页 Hubei Agricultural Sciences
基金 国家自然科学基金项目(31302123) 农业微生物学国家重点实验室开放课题(AMLKF201105)
关键词 猪链球菌2型(Streptococcus SUIS type2 SS2) 1910RR蛋白 原核表达 多克隆抗体 1910RR protein prokaryotic expression polyclonal antibody
分类号 Q78 [生物学—分子生物学]
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参考文献6

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湖北农业科学
2015年 第24期

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