摘要
为了解育种亲本中审定小麦(Triticum aestivum Linn.)品种的品质状况,采用SDS-PAGE技术对127份审定品种高分子量麦谷蛋白亚基(HMW-GS)组成进行分析。结果表明,共发现10种等位基因变异和17种亚基组合类型,其中Glu-A1位点以亚基Null(47.24%)和1(48.03%)为主,Glu-B1位点以7+8(44.09%)和7+9(44.09%)为主,Glu-D1位点只有亚基2+12(70.08%)和5+10(29.92%),总体品质评分为7.12。2002—2011年比1992—2001年审定品种品质有所提升,优质亚基2*、14+15、17+18和5+10出现频率相对提高了,但需注意1亚基频率降低较多。同时,5+10亚基频率仍需提高。另外,还需导入其他亚基种类,以丰富中国小麦HMW-GS组成多样性,提高优质亚基及优质亚基组合频率,改善小麦加工品质。
In order to investigate the quality of wheat varieties as breeding parents, SDS-PAGE technique was used to analyze the HMW-GS composition of 127 wheat varieties. 10 HMW-GS alleles and 17 subunit combination were detected. The subunits Null （47.24%）, 1（48.03%）, 7＋8 （44.09%） and 7＋9 （44.09%） were the major subunits on locus Glu-A1 and Glu-B1. Only two subunits 2＋12 （70.08%） and 5＋10 （29.92%） were detected on locus Glu-D1. The average quality score was 7.12. Comparative analysis of wheat varieties approved from 2002 to 2011 and 1992 to 2001, the frequencies of quality subunits, such as 2＊, 14＋15, 17＋18 and 5＋10, were enhanced. While the frequency of 1 subunit was reduced more often, and should be concerned and improved in future. The frequency of subunit 5＋10 also should be increased too. Meanwhile, the introduction of other subunits to Chinese wheat varieties would abundant the diversity of HMW-GS composition. Through improving the frequencies of quality subunits and combinations, the processing quality of wheat would be improved.
出处
《湖北农业科学》
2015年第24期6330-6333,共4页
Hubei Agricultural Sciences
基金
现代农业产业技术体系项目(CARS-03)
湖北省农业科技创新中心粮食作物分中心资助项目
湖北省农业科学院青年科学基金项目(2014NKYJJ03)
