柴胡加龙骨牡蛎汤对创伤后应激障碍模型大鼠行为学的调节作用

The regulating effect of Chaihu jia Longgu Muli decoction (CLMD) on behavior responds of PTSD rats established by SPS

目的观察柴胡加龙骨牡蛎汤对单次延长应激(single-prolonged stress,SPS)建立的创伤后应激障碍(post traumatic stress disorder,PTSD)模型大鼠行为学的调节作用。方法采用旷场实验以及强迫游泳实验,对不同组大鼠的行为学改变情况进行检测。结果 (1)与正常组大鼠进行比较,模型组大鼠游泳试验不动时间延长、次数增多,旷场实验直立时间减少、运动总距离缩短(P<0.05)。(2)与模型组比较,柴胡桂枝龙骨牡蛎汤以及阿普唑仑均能够降低大鼠游泳试验不动次数、时间以及增加旷场实验直立时间、运动总距离(P<0.05),且两组之间差异无统计学意义(P>0.05)。结论本实验成功复制了PTSD样大鼠模型,柴胡加龙骨牡蛎汤能够改善改变SPS建立的PTSD大鼠的情绪行为变化,能够从总体方面改善PTSD样大鼠的症状。

Objective To investigate the behavior changes and the influence of traditional Chinese prescription Chaihu jia Longgu Mull decoction （CLMD） on post traumatic stress disorder （ PTSD ） rats. Methods The open field test and forced swimming test was used to detect the change of the behavior of rats in different groups. Results （ 1 ） Compared with the normal group, the duration and the times of immobility of model group in forced swimming test were significantly higher （ P 〈 0.05 ）. The vertical activity and total distance movement in the open fields of model group were significantly lower （ P〈0.05 ）. （2） The vertical activity and total distance movement in Xanax group and CLMD group were both significantly higher than those of the model group （ P〈0.05 ）. And the duration and the times of immobility in forced swimming test were significantly reduced in both Xanax group and CLMD group （ P〈0.05 ）. All the parameters of CLMD group and Xanax group had no significant difference（ P〉0.05 ）. Conclusions The PTSD model in rat is established successfully, and CLMD can significantly improve the abnormal behavior changes of the PTSD rats established by SPS model.