蛋白酶水解中药渣制备生物活性肽工艺探讨

Process discussion on preparation of bioactive peptide in Chinese medicine residues by protease hydrolysis

以中成药血必净药渣为材料，研究了通过蛋白酶水解从中制备生物活性肽的工艺．通过考察比较酸性蛋白酶、中性蛋白酶和碱性蛋白酶水解药渣的效果，确定了复合蛋白酶的适宜配比；通过比较不同水解时间的蛋白提取率、水解度和DPPH自由基清除率等指标，确定了复合蛋白酶适宜的水解时间．结果为，选用中性蛋白酶与碱性蛋白酶进行复配来水解制备生物活性肽，适宜配比为3：1，4h为复合蛋白酶适宜的水解时间．在该条件下蛋白质提取率达81％，DPPH自由基清除率达39．8％．

Using the residues of Chinese meicine Xuebijing as materials, bioactive peptide was prepared by protease hydrolysis. The hydrolysis residues effect of acid protease, neutral protease and alkaline protease was investigated. On this basis, the appropriate proportion of compound protease hydrolysis residues was studied, and the suitable hydrolysis time of compound protease was confirmed by comparing the index of different hydrolysis time, such as protein extraction rate, hydrolysis degree and the antioxidant index DPPH free radical clearance rate. The results showed that the neutral protease compounded with alkaline protease was selected to prepare bioactive peptides, appropriate ratio was 3 ： 1 ; the suitable hydrolysis time of complex protease was 4 h, under the condition, the protein extraction rate was 81%, and the antioxidant index DPPH free radical clearance rate was 39.8%.