西柏烯类化合物研究进展

Research advance in cembranoid compounds

对西柏烯类化合物的分离鉴定与含量分析、生物合成与降解、化学合成等方面的研究进展进行了综述.纵观目前出现的分离与分析方法、烟草中西柏烯类化合物的形成与降解等方面的研究发现,柱层析和HPLC法可用于这类化合物的分离与定性定量分析,而GC和GC\|MS方法对这类半挥发性化合物的分析灵敏度较低;烟草中西柏烯类化合物的形成与降解一般认为与光照、氧化和生物催化有关,并进行了大量的研究,但该问题目前尚无定论.未来对西柏烯类化合物的研究应集中在以下几个方面:建立更直接、准确和快速的分离与分析方法;深入开展烟草中西柏烯类化合物经过生物降解形成香味物质的方法研究和该类化合物的形成机制和降解机理研究.

The research progresses on cembranoids, such as separation, identification, qualitative and quantitative analysis, bio-synthesis and bio-degradation as well as chemical synthesis, have been reviewed. Based on the current available separation and analysis methods as well as studies on the formation and degradation of cembranoids in tobacco, column chromatography and HPLC methods have shown good performance on separating and analyzing cembranoids both qualitatively and quantitatively whereas the procedures based on GC and GC-MS methods showed a relatively lower sensitivity to this kind of semi-volatile chemicals. The formation and degradation of cembranoids in tobacco were generally considered to be related to illumination, oxidation and biocatalysis. Many research works done on this aspect, however, no final conclusion has been made. Future research is expected to focus on the following directions： development of more straightforward, accurate and faster analysis methods; in-depth methodological research on biological degradation of cembranoids in tobacco to flavor components; mechanism studies on the formation and degradation of cembranoids