基于DNA链置换反应的圆环形逻辑门设计

Design of circular logic gate based on DNA strand displacement reaction

利用DNA链置换技术反应进程的可编程性和DNA链动力学特征的可预测性,基于DNA链置换反应,以圆环形DNA为基本单元,构造出与非门和或非门逻辑计算模型.该模型以单链DNA分子为输入信号,利用圆环形DNA分子包含的多个DNA识别区域和小支点区域,通过探测荧光信号精确识别其输出信号,来确保DNA分子逻辑门输出结果的正确性与广泛适用性.

Using the DNA strand displacement technology that the reaction progress was programmable and the DNA strand dynamics was predictable, two logic computational models （logic NAND gate and NOR gate） with circular DNA as a basic work unit were designed based on DNA strand displacement reaction. In these models, single-stranded DNA was domains which were contained regarded as the system input. Using many DNA recognition domains and toehold in circular DNA molecule, the output signal was accurately identified by detecting specific fluorescent signal to ensure the correctness and extensive applicability of these DNA logic gate models.