干扰素诱导蛋白16在银屑病患者皮损中的表达及其调控

The Research of the Expression and Regulation of IFI16 in Psoriasis Lesions

目的研究干扰素诱导蛋白16(interferon inducible protein 16,IFI16)在银屑病中的表达及分布,探讨其在银屑病发病中的作用。方法采用RT-PCR、免疫组化的方法分别检测10例银屑病患者皮损和10例健康人皮肤组织真皮与表皮中IFI16的表达情况;采用RT-PCR、Western印记方法检测IFN-γ,TNF-α,IL-17刺激前后Ha Ca T细胞中IFI16的表达情况。结果银屑病患者皮损处IFI16的表达明显高于健康人皮肤,并且以表皮表达为主,免疫组化结果显示IFI16主要定位于银屑病患者的角质形成细胞中。银屑病相关细胞因子IFN-γ,TNF-α和IL-17可以上调角质形成细胞中IFI16的表达水平。结论IFI16在银屑病患者皮损处表达明显高于正常人皮肤,并且IFN-γ,TNF-α和IL-17可以诱导其表达上调,可能是银屑病角质形成细胞的活化机制之一。

Objective To detect the expression and the location of IFI16 in the lesions of psoriasis patients,and discuss the role of IFI16 in the pathogenesis of psoriasis. Methods RT-PCR and immunohistochemistry technique were used to detect the expression of IFI16 in 10 psoriasis lesion and skin tissue from 10 healthy control. RT-PCR and Western blot were performed to investigate the expression of IFI16 in Ha Ca T cells treated with IFN-γ,TNF-α and IL-17. Results We found that IFI16 expression was increased in psoriasis skin lesions,and was located in the epidermis. RT-PCR and Western blot results showed that psoriasisrelated cytokines IFN-γ,TNF-α and IL-17 could induce the overexpression of IFI16 in Ha Ca T cells（ P 〈0. 05）. Conclusion IFI16 expression was increased in the psoriasis lesional skin,and could be induced by IFN-γ,TNF-α and IL-17,which might contribute to the activation of keratinocytes.