内生真菌交织枝顶孢次生代谢产物及其生物活性（英文）

Secondary Metabolites of Endophytic Fungus Acremonium implicatum and Their Biological Activities

从番茄内生真菌交织枝顶孢(Acremonium implicatum)中分离到5个次生代谢产物,经理化特性和波谱数据分析鉴定为对羟基苯甲醛(1)、对羟基苯甲酸(2)、吲哚-3-甲醛(3)、5'-O-乙酰胸苷(4)和酵母甾醇(5)。化合物1和2表现出强的抗细菌、抗真菌、抗氧化和抗线虫活性。化合物3对秀丽隐杆线虫和南方根结线虫表现出一定的抑制活性,半抑制浓度(IC50)分别为82.3μg/m L和93.4μg/m L。结果表明对羟基苯甲醛(1)、对羟基苯甲酸(2)、吲哚-3-甲醛(3)是交织枝顶孢中的主要活性成分,为深入研究交织枝顶孢对线虫的生防机制以及该真菌的开发与应用提供了依据。上述化合物均为首次从交织枝顶孢中分离得到。

Five secondary metabolites were isolated from the ethyl acetate extract of the endophytic fungus Acremonium implieatum from tomato root galls infected with Meloidogyne incognita. They were identified as 4-hydroxy benzaldehyde （ 1 ） ,4-hydroxy benzoic acid （ 2 ）, indole-3-earboxaldehyde （ 3 ）, 5＇-O-acetylthymldine （ 4 ） and eerevisterol （ 5 ） by physieochemical properties and spectrometric methods. Compounds 1 and 2 showed strong antibacterial, antiftmgal, an- tioxidant and antinematodal activities. Compound 3 exhibited moderate antinematodal activity on Caenorhabditis elegans and M. incognita with the median inhibitory concentration （ICso） values of 82.3 Ixg/mL and 93.4 ixg/mL,respective- ly. The results indicated that 4-hydroxy benzaldehyde （ 1 ）, 4-hydroxy benzoic acid （ 2 ）, and indole-3-carboxaldehyde （3） could be the main bioactlve components iB the endophytie fungus A. implicatum. The results will be helpful for clarifying biocontrol mechanisms ofA. implieatum on the root-knot nematode as well as for development and utilization of this fungus. All the compounds were isolated from A. implicatum for the first time.