兖州卷柏水提物对四氯化碳致小鼠肝损伤的保护作用和抗氧化活性（英文）

Hepatoprotective and Antioxidant Activity of Selaginella involvens Aqueous Extract on Carbon Tetrachloride-Induced Hepatic Damage in Mice

研究兖州卷柏对四氯化碳(CCl4)致小鼠肝损伤的保护效果。昆明小鼠随机分为6组(n=8):正常组、CCl4模型组、水飞蓟素对照组、兖州卷柏水提物(高、中、低)剂量组(400、200、100 mg/kg)。各组连续灌胃给药9d后,除正常组外,其余各组用CCl4溶液(2 m L/kg)灌胃。结果显示兖州卷柏能显著的抑制肝损伤血清中SGOT、SGPT、ALP、LDH、胆固醇和胆红素升高(P<0.01)。兖州卷柏能降低损伤肝组织的脂肪含量,局灶性坏死,中心静脉充血和正弦空间拥塞。体外抗氧化实验表明兖州卷柏抗氧化活性指标T-AOC、LPO、T-SOD和NO的IC50值明显高于维生素C(P<0.05)。提示兖州卷柏水提物可能通过其抗氧化活性对四氯化碳致小鼠肝损伤产生保护效果。

In this study,the hepatoproteetive and antioxidant activity of Selaginclla involventis aqueous extract was evalu- ated using carbon tetrachloride （CCl4 ）-induced hepatic damaged mice model. KM mice was randomly divided into 6 groups （n = 8） ,including normal, CCl4-model,Silymarin-control groups and three different dosages （400,200,100 rag/ kg） of S. involventis aqueous extract in experimental groups. After oral gavage for 9 days with drug, KM mice were treated with CC14 （2 mL/kg） by oral gavage,exeept for normal group. The results showed that administration of S. involventis aqueous extracts significandy reduced the level of SGOT, SGPT, ALP, Lactic dehydrogenase （ LDH）, cholesterol and bili- rubin when compared to that of hepatotoxin treated mice. More importantly, histopathologieal data showed that S. invol- ventis aqueous extracts reduced the fatty contents ,focal necrosis ,congestion in central vein and congestion in sinusoidal spaces in CC14-indueed hepatic mice. In addition,the in vitro antioxidant assays demonstrated that the IC5o values of T- AOC, LPO,T-SOD and NO radical scavenging activities were increased when compared with the level of vitamin C. All these experimental results suggested that S. involventis aqueous extract may protect hepatic activity through antioxidant activities in CCl4-indueed mice.