白花蛇舌草半枝莲配伍抗肿瘤作用研究

Anti-tumor Activity of Combination of Hedyotic diffusa and Scutellaria barbata

本研究旨在观察白花蛇舌草和半枝莲配伍对人源乳腺癌细胞的生长抑制作用及其联合环磷酰胺(CTX)对4T1移植瘤的影响。通过制备大鼠含药血清,将人源乳腺癌细胞传代培养后,分别加入药物血清常规培养,采用CCK-8试剂盒检测其细胞存活率;同时建立雌性小鼠移植性乳腺癌细胞(4T1)模型,计算肿瘤抑制率,检测血清TNF-α、INF-γ、IL-2含量(ELISA法)。结果表明CCK-8实验中,给药组含药血清均能显著降低人源乳腺癌细胞的OD值;小鼠移植性乳腺癌实验中,各给药组均可显著提高肿瘤抑制率、血清INF-γ、IL-2含量,下调血清TNF-α水平。配伍组作用明显优于单味药应用。说明白花蛇舌草半枝莲可有效抑制乳腺癌细胞增殖,其中以二药配伍应用效果更佳。

This study investigated the inhibitory effects of combined Hedyotic diffusa and ScuteUaria barbata on human breast cancer cell growth and 4T1 xenografts in mice with cyclophosphamide （CTX）. The human breast cancer cells were cuhured with rat serum containing either the diluent of drug or in combination or CTX. The cancer cell vitality was detected using a CCK-8 kit. In in vivo study,breast cancer cells （4T1） were transplanted to a female mouse, and then was treated as conducted in in vivo experiment. The tumor inhibition rate was calculated by tumor growth, serum tumor necrosis factor-α （TNF-α）, interferon-7 （ IFN-7） and interleukin-2 （IL-2） were detected using ELISA. Results showed that both drugs and CTX significantly reduced the vitality of human breast cancer cells. Similarly in animal model, both drugs and CTX significantly increased tumor inhibition rate, serum INF-γand IL-2 levels, decreased serum TNF-α level. In addition ,the combination of both drugs was more effective on all those parameters compared to each individual. These findings suggested that the combination of H. dlffusa and S. barbata can inhibit cancer cell proliferation more than either of them.