博落回不同部位提取物对大肠菌群的抑菌作用研究

Antibacterial Effect of the Extracts from Different Parts of Macleya cordata Against Coliforms

研究了博落回提取物对大肠菌群的抑制作用。本实验以大肠杆菌、产气肠杆菌、阴沟肠杆菌为指示菌,采用牛津杯法筛选对三种指示菌有最佳抑制作用的博落回组织粗提物;对抑菌效果好的粗提物依次用石油醚、乙酸乙酯、正丁醇进行萃取,采用滤纸片法筛选出抑菌活性最高的萃取物,并测定其最小抑制浓度和最小杀菌浓度。实验结果表明博落回不同部位粗提物有抑制指示菌的活性,其中以博落回根部提取物的抑菌活性最佳;根部萃取物抑菌实验结果表明正丁醇萃取物对三种指示菌均有较好的抑制效果,抑菌圈直径均在12 mm以上,远高于粗提物,对大肠杆菌,其最小抑制浓度和最小杀菌浓度分别为400、600μg/m L,对产气肠杆菌与阴沟肠杆菌,其最小抑制浓度均为500μg/m L,最小杀菌浓度均为700μg/m L。博落回根部提取物对上述三种指示菌均有抑制作用,尤其以正丁醇萃取物的抑菌效果最佳。

In this study, the antibacterial effects of plamepoppy （ Macleaya cordata） extracts against Escherichia coli ,Enterobaeter aerogenes and Enterobacter cloacae were screened by Cylinder-plate method. Subsequently, crude extracts with good antibacterial effect was sequentially extracted with petroleum ether, ethyl acetate and n-butanol. The filter paper method was employed to study the antimierobial activities of these fractions against the tested microorganisms. Moreover, the minimum inhibitory concentration （MIC） and minimum bactericidal eoncentration（MBC） were also studied. The results showed that crude extracts from different parts of M. eordata had antibacterial effect on the tested bacteria, among of which, the extracts of M. cordata roots displayed remarkable inhibition effect. In addition, the n-butanol fractions of M. cordata roots exhibited high antimierobial activities,the inhibition zones were all over 12 ram,which were even better than their crude extracts. The MIC and MBC of E. coli were 400 μg/mL and 600 μg/mL, respectively;while the MIC （ 500 μg/mL） and MBC values （700 μg/mL） were the same for E. aerogenes and E. cloacae. The extracts of M. cordata roots had antibacterial effeets, and the effects of n-butanol fraction were the best.