四种培养液对牦牛孤雌胚胎发育的影响

Effects of four different culture media on parthenogenetic embryo development in yak

为探讨不同培养液对牦牛早期胚胎发育的影响,本试验将经过孤雌激活后的牦牛卵母细胞分别置于m SOF,G1/G2,CR1aa和CR1aa颗粒细胞共培养四种体外培养液中进行体外培养。结果表明,四种培养液对胚胎的卵裂率影响差异不显著,CR1aa和CR1aa颗粒细胞共培养组的囊胚率显著高于m SOF和G1/G2组,CR1aa和CR1aa颗粒细胞共培养组的囊胚率之间没有显著差异,但是CR1aa颗粒细胞共培养组的囊胚孵化率显著高于CR1aa组,说明CR1aa颗粒细胞共培养可作为较为合适的牦牛早期胚胎培养液。

In order to investigate the impacts of different culture media on early yak embryo developmen4 Parthenogenetic activated yak oocytes were divided into four groups and cultured in different in vitro culture media （mSOF, G1/G2, CRlaa and CRlaa granular cell co-culture）. The results showed that cleavage rate among the different groups was not significantly differen4 but blastocyst rate of CRlaa and CRlaa granular cell co-culture group were significantly higher than that of mSOF and G1/G2 group. While blastocyst rate between CRlan and CRlaa granular cell co-culture group showed no significant difference, CRlaa granular cell co-culture group showed a higher hatching rate, suggesting that CRlaa granular cell co-cuhure can be a good candidate for ear ly yak embryo culture.