瑞香狼毒对癫痫大鼠认知功能及海马神经细胞增殖的影响

Proliferation effects of Stellera Chamaejasme Linn. on cognitive function and hippocampal neural cells in epileptic rats

目的探讨狼毒提取物对癫痫大鼠认知及海马神经发生的影响。方法选择健康7周龄雄性SD大鼠,随机分成空白对照组、模型组、高中低剂量实验组、阳性对照组。除空白对照组外,余组腹腔注射氯化锂-匹罗卡品制备动物癫痫模型。高中低剂量实验组腹腔注射氯化锂-匹罗卡品前给予25、50、100 mg的狼毒提取物灌服2周,空白对照组、模型组给予生理盐水,阳性对照组给予苯妥英钠。利用Morris水迷宫实验观察认知功能;用溴脱氧尿嘧啶核苷(Brd U)标记后免疫荧光染色,观察大鼠海马齿状回颗粒细胞层Brd U阳性细胞的增殖情况;以Western-blot技术检测狼毒作用后海马齿状回神经细胞NR1的表达。结果水迷宫结果显示,模型组较狼毒高剂量组潜伏期延长(P<0.05);模型组的Brd U阳性细胞多于对照组(P<0.05),且多于狼毒高剂量组和阳性对照组(P<0.05)。各实验组海马齿状回中神经NR1细胞表达差异无统计学意义(P>0.05)。结论狼毒能改善大鼠的认知功能,且狼毒可干预大鼠癫痫发作后海马齿状回颗粒层Brd U阳性细胞数的异常增生。

Objective To investigate the effects of Stellera Chamaejasme Linn.（ SC） on the change of cognitive function and the proliferation of hippocampal neurons in epileptic rats. Methods Male SD rats（ 7 weeks） were randomly divided into six groups. Vehicle（ blank group and model group）,phenytoin sodium（ positive group） and SC（ 25,50 and 100 mg / kg） were administered via oral gavage. Two weeks after via gavage of test samples,and intraperitoneal injection of lithium- pilocarpine was performed to induce epileptic model in the rats except blank group. After the treatment,the cognitive function was evaluated by Morris water- daze test. The proliferation of neural cells and expression of main proteins in the hippocampal dentate gyrus granular layer were tested by 5- bromodeoxyuridine（ Brd U） labeling and western- blot test,respectively. Results Comparing to model group the escape latency was shortened by SC（ 100 mg/kg） groups when In Morris water- daze test（ P 〈0. 05）. The Brd U positive cells in blank group,positive group and SC（ 100 mg/kg） groups were significantly less than those in control groups（ P 〈0. 05）. There were no significant differences in the expression of main proteins of neural cells in the hippocampal dentate gyrus compared with model group. Conclusion Stellera Chamaejasme Linn. has antiepileptic effect through improving cognitive function and inhibiting the proliferation of Brd U positive cells.