IL-6基因-174G/C位点多态性与宁夏汉族复发性自然流产相关性的研究

Relationship between polymorphism of interleukin-6 gene-174G/C and recurrent spontaneous abortion in Ningxia Han population

目的探讨白细胞介素-6(IL-6)基因启动子-174G/C多态性与宁夏地区汉族女性复发性自然流产(RSA)的相关性。方法应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法,对228例反复自然流产患者和213例健康对照IL-6基因启动子-174G/C多态性位点进行检测。结果 IL-6基因SNP-174G/C的GG、GC基因型在病例组中的频率分布分别为96.93%和3.07%,在对照组中的频率分布分别为97.18%和2.82%,2组的基因型频率分布差异无统计学意义(P>0.05)。IL-6基因SNP-174位点G、C等位基因在RSA组中的频率分布分别为98.46%和1.54%,在对照组中的频率分布分别为98.59%和1.41%,2组的等位基因频率分布差异无统计学意义(P>0.05)。结论 IL-6基因SNP-174G/C位点可能与宁夏地区汉族女性RSA的遗传易感性无关。

Objective To investigate the relationship between polymorphism of interleukin- 6（ IL- 6） gene promoter- 174G/C and recurrent spontaneous abortion. Methods The gene IL- 6- 174 G / C polymorphisms was examined by the polymerase chain reaction- restriction fragment length polymorphism（ PCR- RFLP） method in 228 recurrent spontaneous abortion patients and 213 normal pregnancy women. Results IL- 6 gene at the promoter- 174 G / C the frequencies of GG,GC genotype were 96. 93% and 3. 07% in patients with recurrent spontaneous abortion,while 97. 18% and 2. 82% in healthy controls. No significant difference was seen in two groups（ P 〉0. 05）. IL- 6 gene at the promoter- 174 G / C the allele gene frequency of G and C were 98. 46% and 1. 54% in patients with recurrent spontaneous abortion,while 98. 59% and 1. 41% in healthy controls（ P 〉0. 05）. Conclusion The polymorphisms of IL- 6 gene at the promoter- 174（ G / C） may have no relationship with the genetic susceptibility with recurrent spontaneous abortion in Ningxia female.