倭竹族14-3-3非ε类基因编码区单核苷酸多态性

Single nucleotide polymorphism of non-ε 14-3-3 genes in Shibataeeae

为研究倭竹族Shibataeeae中14-3-3非ε类基因的结构及其编码区单核苷酸多态性,分别从倭竹族5个不同属的代表种中获得14-3-3b,14-3-3c,14-3-3e,14-3-3f的基因全长。这些基因均包含5个外显子和4个内含子,编码序列(CDS)大小为771~789 bp。14-3-3b,14-3-3c,14-3-3e和14-3-3f编码区序列中分别有21,19,26和17个单核苷酸多态性(SNP)位点,SNP频率分别为1SNP/38 bp,1SNP/41 bp,1SNP/30 bp,1SNP/46 bp,基因核苷酸多样性(π)值分别为0.012 17,0.011 83,0.015 21,0.010 38。同一基因在不同竹种间氨基酸序列一致性均达99%以上,毛竹Phyllostachys edulis Pe14-3-3b,Pe14-3-3c,Pe14-3-3e,Pe14-3-3f蛋白的理化性质非常相似,均为亲水性蛋白,含量较多的是丙氨酸(ala),谷氨酸(glu),亮氨酸(leu),等电点为4.75~4.90,这4个蛋白的二级结构也非常相似,α-螺旋结构含量最高,无规则卷曲其次,β-转角和延伸链很少。上述研究为进一步探索竹类植物14-3-3生物学功能奠定基础。

To study the structure and single nucleotide polyrnorphism （SNP） of non-a 14-3-3 in Shibataeeae, homologue genes from five bamboo species, Hibanobambusa tranquillans, Indos^L~a shibataeaoides, Phyl- lost~＇hys edulis, Shibataea chinensis, and Sinobambusa tootsik were cloned with gDNA and cDNA sequences being compared. Results showed five exons and four introns with CDS sequence lengths （in bp） of 789, 771, 789, and 786, respectively. There were 21, 19, 26, and 17 SNP sites in 14-3-3b, 14-3-3c, 14-3-3e, and 14-3-3J coding regions, with SNP frequencies （in bp） of 1SNP/38, 1SNP/41, 1SNP/30, and 1SNP/46, respectively. Nu- cleotide diversity （~＇） for SNP＇s was 0.015 21, 0.012 17, 0.010 98, and 0.009 16, in that order. The amino acid sequence identity for these same genes in different species was 99%. Four 14-3-3 putative proteins were hydrophilic, and their isoelectric point was distributed from 4.75 to 4.90 with the amino acids Ala, Ser, and Gly being abundant. Also, ct-helixs and random coils in these proteins were the most plentiful; whereas, [3-turns and extended strands were few. These results lay a fi}undation for deeper studies with biological functions of 14-3-3 in bamboo. [Ch, 3 fig. 3 tab. 27 ref.]