樟树茎段组培快繁

Tissue culture and rapid propagation for stems of Cinnamomum camphora

以当年生樟树Cinnamomum camphora带芽茎段为外植体,研究6-苄基腺膘呤(6-BA)和萘乙酸(NAA)在初代培养和继代培养过程中对樟树茎段腋芽及丛生芽诱导增殖的影响。结果表明:最适腋芽诱导培养基为MS(Murashige and Skoog)+1.00 mg·L^(-1)6-BA+(0.01~0.10 mg·L^(-1))NAA。继代培养时采用正交试验设计进行培养基筛选,经极差分析和方差分析得知丛生芽发生率较高的培养基为MS+1.00 mg·L^(-1)6-BA+0.10 mg·L^(-1)NAA,而丛生芽增殖最佳培养基为MS+(0.50~1.00)mg·L^(-1)6-BA。将无菌苗转入最适生根培养基1/2MS+1.50 mg·L^(-1) NAA,生根率可达90%;生根的无菌苗移栽至m(蛭石)∶m(泥炭)为1∶1的混合基质中,经过15~20 d练苗,95%以上无菌苗均能成活。这一结果为樟树优良品种的工业化育苗奠定了技术基础。

To study the effect of naphthalene-acetic acid （NAA） and 6-benzylaminopurine 6-BA on the induc- tion and multiplication of multiple shoots for Cinnamomum camphora during the process of primary culture and subculture, stems with axillary buds which sprouted at the beginning of spring were used as explants. To select the optimum medium for subculture, an orthogonal experiment was designed, and an extreme difference analysis and variance analysis （ANOVA） was used. Results showed that the optimal medium for inducing axillary buds was Murashige and Skoog （MS） ＋ 1.00 mg·L-2 6-BA ＋ （0.01-0.10 mg·L-1） NAA. The orthogonal experiment and ANOVA showed that the best medium for formation of multiple shoots was MS ＋ 1.00 mg.L-1 6-BA ＋ 0.10 mg·L-1 NAA, and the optimum medium for proliferation of multiple shoots was MS ＋ 0.50-1.00 mg· L-1 6-BA. After the aseptic seedling was transferred to the optimum rooting medium （1/2MS ＋ 1.50 mg· L-1 NAA）, the rooting rate reached above 90%. After 15-20 d of hardening, seedlings were transplanted to a mixed matrix with equal vermiculite and peat where the survival rate was greater than 95%. Thus, an efficient breeding tech- nology for C. camphora was studied laying a technical foundation for industrialization of improved varieties. [Ch, 2 fig. 5 tab. 13 ref.]