高压氧对缺氧脱习服症大鼠血流动力学的影响

Effects of hyperbaric oxygen on hemodynamics of rats with hypoxia deacclimatization

目的观察高压氧(HBO)对缺氧脱习服症大鼠血流动力学和白细胞介素-6(IL-6)、IL-10、肿瘤坏死因子-α(TNF-α)的影响。方法 SD大鼠在模拟海拔5000 m的低压氧舱内常规喂养3个月,出舱后20只大鼠随机分为HBO组(n=10)、高原对照组(n=10);另外10只在常氧下喂养3个月作为平原对照组。高原对照组出舱后常氧下喂养,HBO组给予HBO治疗,连续7 d。腹腔麻醉后,用多道生理记录仪连续描记大鼠5 min肺动脉压力曲线,测定肺动脉压力(PAP)、左右室收缩压(VSP)和舒张压(VEDP)、右室等容收缩期心室内压力上升最大速率与右室等容舒张期心室内压力下降最大速率比值(±dp/dtmax)等血流动力学指标,并检测血清白细胞介素-6(IL-6)、白细胞介素-10(IL-10)和肿瘤坏死因子-α(TNF-α)、超氧化物歧化酶(SOD)和丙二醛(MDA)水平。结果与平原对照组比较,高原对照组和HBO组左右室VSP、VEDP、±dp/dtmax和m PAP均显著增高(P<0.05或P<0.01);与高原对照组比较,HBO组的上述指标均显著降低(P<0.05或P<0.01)。与平原对照组比较,高原对照组和HBO组SOD值下降(P<0.05),但MDA、IL-6、IL-10和TNF-α均增高(P<0.05或P<0.01);与高原对照组比较,HBO组SOD增高(P<0.05),MDA、IL-6、IL-10、TNF-α降低(P<0.05或P<0.01)。结论高压氧能提高高原脱习服症者机体抗氧化能力,改善组织缺氧损伤后的炎症反应,促进细胞的功能恢复。

Objective To observe the effects of hyperbaric oxygen （HBO） on the hemodynamics and interleukin-6, interleukin- 10, and TNF-α of rats with hypoxia deacclimatization. Methods SD rats had been housed in a hypobaric hypoxia chamber at the simulated altitude of 5000 m for 3 months. After that, twenty SD rats were randomly and equally divided into HBO group and high altitude control group. Another 10 had been raised in normal pressure and oxygen environment as the plain control group for 3 months. The high altitude control group had been raised in normal pressure and oxygen environment for 7 d after hypobaric hypoxia exposure, and the HBO group received the hyperbaric oxygen treatment for 7 d. After the intraperitoneal anesthesia, polygraph was used to continuously trace the five-minute pulmonary artery pressure curve, and detection was made in the pulmonary artery pressure （PAP）, left and right ventricular systolic pressure （VSP） and diastolic blood pressure （VEDP）, the ratio of the fight ventricular systolic ventricular isovolumic maximum pressure rise rate to the right ventricular diastolic ventricular isovolumic maximum pressure drop rate （＋dp/dtmax）, and other hemodynamic parameters. And detection was also made in the IL-6, IL-10, TNF-α, superoxide dismutase （SOD）, and malondialdehyde （MDA） levels,Results Compared with the parameters in the plain control group, the left and right VSP, VEDP, ＋dp/dtmax, and mPAP in the high altitude control group and HBO group significantly increased （P 〈 0.05 or P 〈 0.01）. Compared with those parameters in the high altitude control group, those in the HBP group all significantly decreased（P 〈 0.05 or P 〈 0.01）. Compared with the SOD, MDA, IL-6, IL-10, and TNF-α in the plain control group, the SOD in the high altitude control group and the HBO group decreased （P 〈 0.05）, while other parameters increased （P 〈 0.05 or P 〈 0.01）. Compared with the SOD, MDA, IL-6, IL-10, and TNF-α in the high altitude control group, the SOD in the HBO group increased （P 〈 0.05）, while other parameters decreased （P 〈 0.05 or P 〈 0.01）. Conclusion Hyperbaric oxygen can improve the oxidation resistance ability and the inflammatory reaction after hypoxia injury and effectively promote cell functional rehabilitation.