跑台训练对大鼠脑缺血再灌注损伤后胶质纤维酸性蛋白和脑源性神经营养因子表达的影响

Effects of Treadmill Training on Expression of Glial Fibrillary Acidic Protein and Brain- derived Neurotrophic Factor in Rats with Cerebral Ischemia-reperfusion

目的观察跑台训练对大鼠脑缺血再灌注损伤后胶质纤维酸性蛋白(GFAP)和脑源性神经营养因子(BDNF)表达的影响。方法成年雄性Wistar大鼠30只随机分为假手术组、模型组和跑台训练组,每组10只。采用线栓法制备大脑中动脉阻塞2 h再灌注模型。假手术组插线10 mm后即刻退出。跑台训练组在造模成功后第3天进行跑台训练12 d,在造模后第4、8、15天采用改良神经功能缺损评分(m NSS)对各组大鼠评分,造模后第15天HE染色观察脑组织病理学变化,Western blotting检测BDNF和GFAP表达。结果造模后15 d,与模型组比较,跑台训练组m NSS评分明显降低(F=9.931,P<0.01),缺血侧皮质脑组织病理损伤减轻,GFAP(t=6.73)和BDNF(t=3.78)表达明显升高(P<0.01)。结论跑台训练可促进大鼠脑缺血再灌注损伤后GFAP和BDNF的表达,促进神经功能的恢复。

Objective To explore the effect of treadmill training on expression of glial fibrillary acidic protein （GFAP） and brain-derived neurotrophic factor （BDNF） after cerebral ischemia-reperfusion in rats. Methods 30 male Wistar rats were randomly divided into sham group, model group and treadmill training group, with 10 rats in each group. The latter 2 groups were modeled with middle cerebral artery occlusion for 2 hours and reperfusion. The treadmill training group underwent treadmill exercise on the 3rd day after modeling for 12 days. Neurological function was evaluated with modified Neurological Severity Scores （mNSS）. The neuronal pathological change in ischemic cortex was observed with HE staining. The expressions of GFAP and BDNF in cortex were determined by Western blotting. Results Compared with the model group, the mNSS scores decreased in the treadmill training group （F=9.93 l, P〈0.01 ）, the pathological damage in the ischemia cortex significantly lessened, and the expressions of GFAP （t=-6.73） and BDNF （t=3.78） increased （P〈0.05）. Conclusion Treadmill training may increase the expression of GFAP and BDNF after cerebral ischemia-reperfusion in rats, and promote the recovery of neurological function.