摘要
目的:探讨百里醌抑制体外骨肉瘤细胞转移的作用及其机制。方法:本研究应用不同浓度百里醌作用人骨肉瘤Sa OS-2细胞后,观察百里醌对体外骨肉瘤Sa OS-2细胞迁移和侵袭的作用;Western blotting检测骨肉瘤细胞中MMP-2和MMP-9表达的变化。结果:百里醌可呈浓度依赖性抑制体外人骨肉瘤骨肉瘤Sa OS-2细胞迁移和侵袭,同时百里醌可下调骨肉瘤Sa OS-2细胞中MMP-2和MMP-9的表达。结论:百里醌可抑制体外骨肉瘤细胞转移,该作用可能通过下调MMP-2和MMP-9的表达而实现。
Objective: To investigate the anti-metastasis effect and its mechanism of thymoquinone on the osteosarcoma SeeS-2 cell line in vitro.Method: In the study, after application of different concentrations of thymoquinone in the role of human osteosarcoma SaOS-2 cells, the effects of thymoquinone on the migration and invasion of osteosarcoma SeeS-2 cells were examined by using wound assay and matrigel counting.Western blotting was used to detect the protein expression of MMP-2 and MMP-9 in SeeS-2 cells.Result: Thymoquinone significantly suppressod the migration and invaaion of osteosarcoma SaOS-2 cells in a concentration depended manner,The expression of MMP-2 and MMP-9 were down-regulated in osteosarcoma SaOS-2 cells after treatment of thymoquinone,Conclusion: Thymoquinone exerts anti-metastatic activity in SaOS-2 cells in vitro, which may be achieved by down-regulating the expression of MMP-2 and MMP-9.
出处
《中国医学创新》
CAS
2016年第5期26-29,共4页
Medical Innovation of China
关键词
骨肉瘤
百里醌
转移
Osteosarcoma
Thymoquinone
Metastasis