摘要
近年来，纳秒脉冲技术的临床应用研究不断深入与发展，但是其治疗的细胞学机制至今仍未完全阐明。在学术界存在各种假说和理论。研究证实，纳秒脉冲电场可以穿透细胞膜形成纳米孔，引起钙离子内流；也可作用于细胞内膜，引起内质网损伤、线粒体膜电位差的改变；进一步还可以损伤细胞微丝骨架，导致细胞形态的变化。本文结合最新的研究进展，围绕纳秒脉冲电场对细胞膜和细胞器的分子作用机制进行介绍。
Nanosecond pulsed electric field ablation has been widely applied in clinical cancer treatment, while its molecular mechanism is still unclear. Researchers have revealed that nanosecond pulsed electric field generates nanopores in plasma membrane, leading to a rapid influx of Ca^2＋; it has specific effect on intracellular organelle membranes, resulting in endoplasmic reticulum injuries and mitochondrial membrane potential changes. In addition, it may also change cellular morphology through damage of cytoskeleton. This article reviews the recent research advances on the molecular mechanism of cell membrane and organelle changes induced by nanosecond pulsed electric field ablation.
出处
《浙江大学学报（医学版）》
CAS
CSCD
北大核心
2015年第6期678-683,共6页
Journal of Zhejiang University(Medical Sciences)
基金
国家自然科学基金(81372425)
浙江省自然科学基金(LY13H180003)
新疆维吾尔自治区重点实验室专项资金(2014KL002)
关键词
肿瘤/治疗
电穿孔
辐射
电离
激光
细胞/辐射效应
综述
Neoplasms/therapy
Electroporation
Radiation, ionizing
Lasers
Cells/radiation effects
Review
