脉冲场凝胶电泳技术在致病菌分型技术的应用

Pulsed-field Gel Electrophoresis and its Application in Pathogen Molecular Typing

脉冲场凝胶电泳（pulse-field gelelectrophoresis,PFGE）,又称脉冲式交变电场电泳,该技术是将电泳电场方向交替性改变,并优化脉冲时间和其他条件,可以分辨凝胶中35~10 000 kb的DNA分子。该方法应用于分子流行病学中,通过观察电泳条带的差异,为确定菌株之间的亲缘关系提供了可靠的技术手段。该技术在致病菌溯源（细菌性传染性疾病溯源、细菌性食源性疾病溯源）、医院内感染流行监测等方面都有广泛的应用。

Pulsed Field Gel Electrophoresis （PFGE） is a new technique of gelelectrophoresis developed in1980＇s. It can be used to separate large DNA molecules ranging from 35 to10 Mb. It has been widely applied to the pathogen molecular typ-ing , identification of species groups ,Source-tracking of pathogen and nosocomial infection surveillance.