STAT4、STAT6在子痫前期胎盘组织中的表达及意义

The expressions of STAT4 and STAT6 in placenta of preeclampsia patients and its significance

目的:探讨子痫前期患者胎盘组织中信号转导和转录激活因子(STAT)4以及STAT6的表达及意义。方法:采用免疫组织化学链霉菌抗生物素蛋白-过氧化物酶连接(SABC)法和Western blot法检测30名正常妊娠晚期孕妇(对照组)和50例子痫前期患者(子痫前期组,其中轻度22例、重度28例)胎盘组织中STAT4、STAT6的蛋白定位和表达。结果:STAT4主要表达于绒毛滋养细胞的胞质,胞核多数无明显着色;轻、重度子痫前期组胎盘组织中STAT4的表达均明显高于对照组(P<0.01);STAT6主要表达于绒毛滋养细胞的胞质,胞核多数无明显着色;轻度、重度子痫前期组均显著低于对照组(P<0.01),且轻、重度子痫前期组STAT4和STAT6表达水平差异均有统计学意义(P<0.01)。子痫前期组和正常妊娠组绒毛组织中STAT4与STAT6的表达水平均呈负相关关系(r=-0.509,r=-0.617;P<0.01)。结论:STAT4、STAT6共同参与子痫前期的病理生理改变,并与病情严重程度有一定关系。

Objective： To investigate the signal transduction and expression of STAT4 and STAT6 in placenta of preeclampsia patients and its significance. Methods： The expressions and location of STAT4 and STAT6 in placenta of 30 normal late pregnant women（ control group） and 50 preeclampsia patients（ including 22 mild cases and 28 severe cases） were observed using Western blot and immunohistochemistry,respectively. Results： The expressions of STAT4 and STAT6 located mainly in the cytoplasm of villous trophoblast cell,the most nucleus were no clear coloring. The expressions of STAT4 and STAT6 were significantly higher and lower than those of control group,respectively（ P〈0. 01）. The differences of the expressions between STAT4 and STAT6 in placenta of mild and severe preeclampsia patients were statistically significant（ P〈 0. 01）. The expressions of STAT4 in the cytoplasm of villous trophoblast cell in two groups were negative correlation with the level of STAT6（ r =- 0. 509,r =- 0. 617; P〈0. 01）. Conclusions： Both STAT4 and STAT6 paticipate the pathogenic process of preeclampsia,and are related to the severe degree of disease. The high expression of STAT4 and low expression of STAT6 in the villous trophoblast cell of preeclampsia patients can lead to Th1 / Th2 imbalance,which may lead to the preeclampsia.