梅毒联合检测避免酶联免疫吸附试验假阴性分析

目的了解常用梅毒临床检测方法的优缺点，减少因方法学缺陷造成的误差。方法将患者用酶联免疫吸附试验（ELISA）方法检测的原倍量假阴性血清倍比稀释后，再用同样方法检测。结果得到的数据验证了患者血清中存在极高浓度的梅毒特异性抗体，这是造成检测结果假阴性误差的原因。结论梅毒ELISA检测在极个别情况下因患者体内的特异性抗体浓度超高，引起前带现象导致假阴性结果的出现，提示单一梅毒检测方法在某些N素影响下会导致结果的假阳性或假阴性．此情况应引起高度重视。

Objective Learn the advantages and disadvantages of syphilis clinical detection methods,Reduce errors caused due to methodological shortcomings.Methods The original times the amount of false negative serum with ELISA （enzyme-linked im- munosorbent assay） method for the detection after dilution with the same method to detect.Results The data obtained to verify the presence of high concentrations of specific antibodies in the serum of patients with syphilis,it is the cause of false-negative error detection result.Concluslon ELISA detection of syphilis in rare cases due to patient-specific antibody concentration ultra- high,former band phenomenon causes leading to false negative results occur,prompt us to single syphilis testing method under the influence of certain factors that could cause the results of false positive or false negative,this situation should arouse our attention~