邻苯二甲酸二丁酯高效降解菌的分离、鉴定及降解特性

Isolation and identification of highly efficient DBP-degrading strain and its degradation study

从土壤中分离出1株能够以邻苯二甲酸二丁酯为碳源和能源生长的细菌XHYG.经形态观察、生理生化鉴定、16S rDNA序列及系统发育分析,鉴定该菌株为无色杆菌（Achromobacter insolitus）.对该菌株的降解条件进行优化,确定最佳降解条件为：温度30℃,p H=6.5~8.0.在最佳降解条件下,其在48 h内对400 mg/L DBP降解率达到90.67%,为邻苯二甲酸二丁酯的高效降解菌.底物降解广谱性试验表明,该菌株对邻苯二甲酸二辛脂（DOP）、邻苯二甲酸（2-乙基已基）酯（DEHP）都具有良好的降解能力,表明具备良好的底物降解广谱性,说明该菌株在处理邻苯二甲酸酯类化合物的污染治理中有独特的应用潜力.

A bacterial strain XHYG which can utilize Dibutyl phthalate（ DBP） as the source of carbon and energy was isolated from soil. Based on its morphology,physiological and biochemical identification,and 16 S rDNA sequence analysis,XHYG was identified as Achromobacter insolitus. The results also showed that the optimum temperature and p H for its growth and styrene-degradation were 30℃ and 6. 5 ~ 8. 0,respectively. Under the optimal conditions,strain XHYG degraded more than 90. 67% of 400 mg / L DBP within 48 h. Diversity of degradable substrates also showed that HS-B1 can efficiently utilize many other phthalate esters such as di-n-octyl phthalate（ DOP） and bis（ 2-ethylhexyl） phthalate（ DEHP）. It revealed that the strain XHYG has special application potential in dealing with the pollution caused by phthalate esters.