一种病证结合痰浊阻窍证老年期痴呆大鼠模型的建立

Establishment of the Combined Disease and Syndrome Model of Phlegm Blockage in Senile Dementia Rats

目的:建立一种病证结合的痰浊阻窍证老年期痴呆大鼠模型。方法:61只大鼠随机分为A空白对照组、B老年性痴呆组、C血管性痴呆组、D痰浊阻窍证组、E病证结合(AD+VD+痰浊阻窍证)组,从Morris水迷宫、血脂、海马神经元超微结构等不同方面,对痰浊阻窍证老年期痴呆大鼠模型的建立进行初步探索。结果:造模成功后,Morris水迷宫实验显示B、C、E组与A组间逃避潜伏期及目标象限停留时间、原平台区穿越次数数据,差异有统计学意义。血脂化验显示,D组及E组的TC、LDL-C升高为其余各组的2倍以上。透射电镜下观察大鼠海马CA1区超微结构,可见E组的神经元细胞超微结构受损最为严重,B组次之,再次为C组与D组。结论:该模型可作为痰浊阻窍证老年期痴呆模型使用,用于老年期痴呆的实验研究。

The syndrome of phlegm obstructing orifices of senile dementia rat model was estabhshed by a combination of disease and syndrome. Methods： 61 rats were randomly divided into A blank control group, BAD group, C VD group, D phlegm blocking orifices group, E combination of disease and syndrome （AD ＋ VD ＋ syndrome of phlegm obstructing orifices） group, from the behavior of rats, biochemical, morphological aspects preliminary exploration of establishing model, the syndrome of phlegm obstructing orifices of senile dementia rats. Results： After the success of modeling, Morris water maze test showed that B, C, E group and A group had significant difference. Blood tests： D group and E group TC, LDL-C increased above 2 times ＋ the other groups. Observation on ultrastructure of hippocampus CA1 area of rats under transmission electron microscope, neuronal cell uhrastructure was observed in E group the damage was most severe, followed by the B group, C group and D group again. Conclusion： The model may be used as phlegm blocking orifices of senile dementia model, used for experimental study of senile dementia.