TGF-β1-509C/T基因多态性与不同中医证型原发性膝骨关节炎的关系

Association of TGF-β1-509C/T polymorphisms with Common Traditional Chinese medicine Syndromes primary knee osteoarthritis

目的探讨TGF-β1-509C/T基因多态性与不同中医证型原发性膝骨关节炎（primary knee osteoarthritis,PKOA）遗传易感性的关系。方法采用关联分析,严格按照病例纳入标准,随机选取湖南地区PKOA患者88例（瘀血阻滞组52例,肾虚亏髓组36例）,应用聚合酶链式反应-限制性片段长度多态性分析（PCR-RFLP）对所选对象TGF-β1-509位点基因和基因型进行检测。结果瘀血阻滞型PKOA组携带CC基因型的频率明显要高于对照组（50.0%vs 13.5%;χ~2=15.996,P〈0.05）,差异有统计学意义;Logistic回归分析结果显示,与TGF-β1-509位点TT基因型相比,携带TGF-β1-509位点CC基因型有患瘀血阻滞型PKOA风险（OR=9.75,95%CI=7.10-13.46））。结论 TGF-β1-509位点与瘀血阻滞型PKOA相关,CC基因型是瘀血阻滞型PKOA易感基因型。

Objective To probe association of the promoter -509C/T gene polymorphisms of transforming growth factor-β1 （TGF-β1） with Common raditional Chinese medicine Syndromes primary knee osteoarthritis （PKOA）. Methods the promoter - 509C/T gene polymorphisms were analysised by polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） in 88 patients with primary knee osteoarthritis （qi stagnation and blood stasis syndrome 52,Kidney empty marrow deficient syndrome36） from Affiliated Hospital to Hunan Insititute Chinese Medicine. Results Blood stasis type PH group carrying CC genotype frequency is significantly higher than the control（50.0% vs 13.5%;χ^2=15.996 ,P〈0.05）,the difference was statistically significant; Logistic regression analysis showed that the TGF compared -β1-509 sites TF genotype,TGF-β1-509 locus genotype CC suffering from qi stagnation and blood stasis syndrome PKOA risk （OR=9.75,95%CI=7.10-13.46）. Conclusion the promoter - 509C/T gene polymorphisms of transforming growth factor-β1 is associated with the PKOA patients. The CC genotype may be qi stagnation and blood stasis syndrome risk genotype.