电针穴位对受辐照小鼠认知功能和学习记忆的影响

Effects of electroacupuncture on cognitive function and learning and memory of mice exposed to ionizing radiation

目的 观察电针百会、风府和双侧肾俞穴位对射线照射模型小鼠认知及空间学习记忆能力的影响.方法 100只30 d龄C57BL/6J小鼠按随机数字表法分成对照组、模型组（4、8和16 Gy）、电针非穴位组（4、8和16 Gy）、电针穴位组（4、8和16 Gy）,每组10只.不同剂量射线照射（4、8和16 Gy）建立小鼠放射性脑辐射损伤模型,电针治疗3个疗程.测定小鼠体重进行一般状况评价,旷场实验测试小鼠认知功能,Morris水迷宫实验测试空间学习记忆能力.结果 照后7和14d,与对照组比较,模型组（8和16 Gv）小鼠体重明显减少（￡=2.917、9.650、3.043、2.882,P＜0.05）;与模型组（8和16 Gy）比较,电针穴位组小鼠体重明显增加（t=6.602、5.409、19.660、2.975,P＜0.05）.在旷场实验中,与对照组比较,模型组（8和16 Gy）小鼠垂直运动和水平运动次数显著减少（￡=2.183、2.119、2.369、2.231,P＜0.05）;与模型组（8和16 Gy）和电针非穴位组（8和16 Gy）小鼠比较,电针穴位组小鼠垂直运动和水平运动显著性增加（=8.914、2.727、4.325、4.178,P＜0.05;￡=4.030、3.014、2.150、4.771,P＜0.05）.Morris水迷宫实验中,与对照组比较,在测试第3天,模型组（4 Gy）小鼠潜伏期明显延长,而穿越靶象限次数减少（=4.544、3.422,P＜0.05）;与模型组（4 Gy）及电针非穴位组（4 Gy）比较,电针穴位组（4 Gy）潜伏期显著缩短（t=2.877、3.001,P＜0.05）.与对照组比较,在测试第2、3天,模型组（8和16 Gy）小鼠潜伏期显著延长（t==2.544、3.706、3.796、7.934,P＜0.05）,穿越次数减少（=2.120、2.393,P＜0.05）,模型组（8 Gy）停留时间减少（=2.543,P＜0.05）.与模型组（8和16 Gy）和电针非穴位组（8和16 Gy）小鼠比较,电针穴位组小鼠潜伏期显著缩短（与模型组比较：t==5.404、7.869、4.104、15.590,P＜0.05;与电针非穴位组比较：=2.938、7.955、6.566、10.350,P＜0.05）,而穿越靶象限次数和停留时间仅电针穴位组（8 Gy）显著增加（与模型组比较：t=2.673、2.613,P＜0.05;与电针非穴位组比较：t=3.345、2.179,P＜0.05）.结论 8 Gy以上射线照射可建立脑辐射损伤模型,电针（百会、风府和双侧肾俞）能够显著改善模型小鼠认知能力及空间学习记忆能力.

Objective To investigate the effects of electroacupunctures at Baihui,Fengfu,and Shenshu on the cognitive function and learning memory of mice exposure with ionizing radiation.Methods C57BL/6J （30-day old） mice were randomly divided into l0 groups consisting of the control group,X-ray exposure groups （4,8 and 16 Gy）,electroacupuncture-nonacupiont （E-nonA） groups （4,8 and 16 Gy） and electroacupuncture-acupiont （EA） groups （4,8 Gy and 16 Gy）,with 10 mice assigned to each group.The mice were irradiated （IR） on the left cerebral hemisphere with doses of 4,8 and 16 Gy.Some mice were electroacupunctured for 3 courses of treatment.Using weight as a measurable parameter,the open-field test and Morris water maze test were used to evaluate the curative effects.Results Compared with the control group,the weights of mice in the exposure groups （8 and 16 Gy） significantly decreased （t =2.917,9.650,3.043,2.882,P 〈 0.05） at 7 and 14 d,while the weights of the E-nonA （8 and 16 Gy） and EA groups （8 and 16 Gy） were not significantly altered.Compared to the model groups （8 and 16 Gy）,the weights of EA groups （8 and 16 Gy） significantly increased （t =6.602,5.409,19.660,2.975,P 〈 0.05）.In the open-field test,the crossing times and the rearing time of exposure model groups （8 and 16 Gy） significantly decreased compared to the control group （t =2.183,2.119,2.369,2.231,P 〈 0.05）.Compared with model groups （8 and 16 Gy） and E-nonA groups （8 and 16 Gy）,the crossing times and rearing time of the EA groups were significantly increased （compared with model：t =8.914,2.727,4.325,4.178,P 〈 0.05;compared with E-nonA：t =4.030,3.014,2.150,4.771,P 〈0.05）.In the Morris water maze,compared with the control,the latent period of model group （4 Gy） was significantly increased and the crossing times significantly decreased （t =4.544,3.422,P 〈 0.05）;compared to model and E-nonA groups （4 Gy）,the latent period of EA group significantly increased （compared with model：t =2.877,P 〈 0.05;compared with E-nonA：t =3.001,P 〈 0.05）.At day 2 and 3,the latent period of model groups （8 and 16 Gy） significantly increased （t =2.544,3.706,3.796,7.934,P 〈 0.05） and the crossing times （t =2.120,2.393,P 〈 0.05） significantly decreased,resident time in target area of model group （8 Gy） was significantly decreased （t =2.543,P 〈 0.05） compared to the control group.Compared with model and E-nonA groups （8 and 16 Gy）,the latent period of EA gourps significantly decreased （compared with model：t =5.404,7.869,4.104,15.590,P 〈 0.05;compared with E-nonA：t =2.938,7.955,6.566,10.350,P 〈 0.05） while the through times and resident time in target area significantly increased in the EA group （8 Gy） （compared with model：t =2.673,2.613,P 〈 0.05;compared with E-nonA：t =3.345,2.179,P 〈 0.05）.Conclusions Irradiation with a dose higher than 8 Gy could induce mice brain injury.Electroacupuncture can alleviate the radiation damage of cognitive function as well as learning and memory functions in mice.